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PXD012945

PXD012945 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFree mRNA interactome upon heat shock in Hela cells
DescriptionThe polypeptide-coding mRNAs are coated by specialized proteins at any stage of their lifecycle. One of few circumstances when free mRNA appears in the cytosol is the disassembly of polysomes during stress-induced shutdown of protein synthesis. Using quantitative mass spectrometry, we identified free RNA interactors in the heat-shocked mammalian cells and reconstituted the protein-RNA association in vitro. RNA-associated proteins displayed higher disorder and larger size, which supports the role of multivalent interactions during the initial phase of the RNA granule formation. Structural features of the free RNA interactors defined them as a subset of RNA-binding proteins. Our results reveal how free RNA can participate in reorganization of cellular functions during proteostasis stress.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:53:43.101.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGiulia Calloni
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-04 01:56:18ID requested
12019-06-28 05:29:51announced
22024-10-22 04:53:43announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.9b00143;
Alriquet M, Mart, í, nez-Lim, ó, n A, Hanspach G, Hengesbach M, Tartaglia GG, Calloni G, Vabulas RM, Assembly of Proteins by Free RNA during the Early Phase of Proteostasis Stress. J Proteome Res, 18(7):2835-2847(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: stress granules,mRNA, proteostasis stress, RNA granules
Contact List
R. Martin Vabulas
contact affiliationBuchmann Institute of Molecular Life Sciences and Institute of Biophysical Chemistry, Goethe University, Frankfurt am Main, Germany
contact emailvabulas@em.uni-frankfurt.de
lab head
Giulia Calloni
contact affiliationGoethe University Frankfurt
contact emailcalloni@em.uni-frankfurt.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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