PXD012944

PXD012944 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Candida albicans after serial systemic infection in murine model
Description	This study assessed proteomic profile of Candida albicans after serial systemic infection in a murine model. The animals were infected initially by wild-type C. albicans SC5314 (WT) with an inoculum of of 3.5x105 cells via lateral tail vein. Then, five days post-infection, the animals were euthanized and their kidneys were removed, homogenized in lysis buffer, plated on SDA and incubated for 24 h at 35 °C. Colonies recovered from infected kidney were used to prepare inoculum for the subsequent infections as described for WT, totalizing five serial passages (P1-P5) and they were also used to protein extraction. By LC-MS/MS, 479 proteins were identified, with 56 proteins statistically significant in abundance in P1, 29 proteins in P3 and 97 proteins in P4. Regarding biological processes, the majority of proteins were related to carbohydrate metabolism, stress response and amino acid metabolism. The proteins were also categorized according to their potential role in virulence factors such as biofilm production, yeast-to-hyphae transition, phenotypic switching, proteins related to stress response and uncharacterized proteins. Therefore, serial infection associated with proteomic approach enabled to deepen the knowledge about host-pathogen interaction.
HostingRepository	PRIDE
AnnounceDate	2019-07-01
AnnouncementXML	Submission_2019-07-15_07:31:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Glaucia Arita
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-04 01:42:47	ID requested
1	2019-07-01 00:35:31	announced
⏵ 2	2019-07-15 07:31:48	announced	Updated publication reference for PubMed record(s): 31293987.

Arita GS, Meneguello JE, Sakita KM, Faria DR, Pilau EJ, Ghiraldi-Lopes LD, Campanerut-S, á PAZ, Kioshima É S, Bonfim-Mendon, ç, a PS, Svidzinski TIE, Infection Highlighted by Proteomics. Front Cell Infect Microbiol, 9():230(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Candida albicans, systemic candidiasis, serial passage, virulence, host–pathogen interaction, proteomics, mass spectrometry, LC-MS/MS

Patrícia de Souza Bonfim de Mendonça
contact affiliation	Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringá, Brazil
contact email	psbmendonca2@uem.br
lab head
Glaucia Arita
contact affiliation	Universidade Estadual de Maringá
contact email	glauciasayuria@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944

PRIDE project URI

• PRIDE
  1. PXD012944
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Candida albicans after serial systemic infection in murine model