PXD012940

PXD012940 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of clinically relevant proteins in modern and traditional bread wheat cultivars
Description	Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for the human consumption. Its grain storage proteins greatly impact bread quality, though may cause food intolerances or allergies in susceptible individuals. Consequently, the investigation of a proteome polymorphism among wheat varieties is important to spot the genotypes, which would be promising donors for the breeding of hypoallergenic cereals. Herein, we discovered diversity of grain proteins in three Ukrainian wheat cultivars: ‘Sotnytsia’, ‘Panna’ (both modern selection) and ‘Ukrainka’ (old landrace). Firstly, proteins were isolated with a SDS-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins and albumins). Secondly, the proteome was profiled by the two-dimensional gel electrophoresis, revealing 810 clearly-separated gel spots. Software-assisted analysis of gel images, showed 66 differentially abundant proteins. Using multi-enzymatic digestion, followed by the tandem mass spectrometry, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography profiling and direct mass spectrometry quantification complemented the results. With this approach we quantified 127 proteins, 12 being differentially abundant. Principal component analysis confirmed genotype as a major source of variation in both cases. Non-gluten fraction was the most diverse among investigated bread wheat cultivars. Information from public databases of clinically relevant plant proteins highlighted variable groups of wheat allergens/toxins. Data suggested that one of the modern cultivars contained less health affecting proteins in grain. Finally, we proposed set of genetic landmarks for the development of DNA marker system, which will enable fast and efficient assessment of medical safety of multiple wheat genotypes to facilitate breeding programs.
HostingRepository	PRIDE
AnnounceDate	2020-05-20
AnnouncementXML	Submission_2020-05-19_22:54:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012940
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	SYNAPT G2-Si; Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-04 01:36:43	ID requested
⏵ 1	2020-05-19 22:54:26	announced

Lakhneko O, Danchenko M, Morgun B, Kov, á, č A, Majerov, á P, Š, kult, é, ty Ľ, Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars. Int J Mol Sci, 21(10):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Triticum aestivum, food quality, cereal allergens, grain, two-dimensional gel electrophoresis, mass spectrometry

Andrej Kovac
contact affiliation	Institute of Neuroimmunology, Slovak Academy of Sciences
contact email	andrej.kovac@savba.sk
lab head
Maksym Danchenko
contact affiliation	Institute of Virology, Biomedical Research Center
contact email	maxymdan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012940
     1. Label: PRIDE project
     2. Name: Comparative proteomics of clinically relevant proteins in modern and traditional bread wheat cultivars