PXD012936

PXD012936 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative Proteomics Analysis of lycorine treatment in highly pathogenic avian influenza H5N1 virus infection
Description	Highly pathogenic avian influenza virus (HPAIV) is a high morbidity-and mortality-associated disease that causes rapid systemic illness and death in susceptible animals. Lycorine, as the major component of Amaryllidaceae alkaloids, exhibits better protective effects against A/Chicken/Guangdong/178/2004 (GD178) than the commercial neuraminidase (NA) inhibitor oseltamivir in our prior study. Although the change of proteins of the whole cell in response to HPAIV infection have been revealed by proteomics, the mechanisms resulted in lycorine-related symptoms and the crucial factors remain to be elucidated. In this study, we carried out Multiplex tandem mass tag (TMTs) approach to analyze protein level variation of virus-infected host cells treated by lycorine using. Three groups were administrated: mock infection group (L), virus infection group (M) and virus infection and lycorine-treated after virus infection group (H). 5786 proteins were identified, among which from these three groups using TMT proteomics analysis. In M/L group, 1101 proteins were identified, of which 340 proteins were determined differentially expressed proteins during HPAIV; meanwhile, 1059 proteins were identified from lycorine-treated group, among which 259 proteins presented significant change. Here, proteins in M/L and M/H were acquired simultaneously trend, and the proteins in each fraction were analyzed. Through the enrichment analysis of Gene Ontology (GO)and KEGG pathways, we found that the up-regulated proteins in the mitogen-activated protein kinase signaling pathway and RNA transport were most enriched after HPAIV infected. There were 70 differentially abundant proteins with co-changed proteins in M/L and M/H groups were selected as the candidate proteins. Moreover, we noticed that the nuclear pore complex protein (Nup) 93 was associated with both mitogen-activated protein kinase signaling pathway and RNA transport. In addition, the Western blot experiments confirmed that the expression of Nup93 was significantly down-regulated in lycorine treatment but induced after viral infection. Conclusion: Thus, the research may provide new insight into lycorine’s underlying anti-influenza mechanisms and potential therapeutic targets for influenza virus
HostingRepository	iProX
AnnounceDate	2019-08-27
AnnouncementXML	Submission_2023-03-01_19:45:44.963.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Zhou
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	TMT6plex reporter fragment
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-03 23:34:31	ID requested
1	2019-08-26 20:59:51	announced
⏵ 2	2023-03-01 19:45:45	announced	2023-03-02: Update publication information.

Yang L, Zhang JH, Zhang XL, Lao GJ, Su GM, Wang L, Li YL, Ye WC, He J, Tandem mass tag-based quantitative proteomic analysis of lycorine treatment in highly pathogenic avian influenza H5N1 virus infection. PeerJ, 7():e7697(2019) [pubmed]

submitter keyword: Highly pathogenic avian influenza virus

Proteomics

Lycorine

Nup93

Jun He
contact affiliation	Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University
contact email	hejun@jnu.edu.cn
lab head
Qian Zhou
contact affiliation	Shanghai Omicsspace Biotech Co., Ltd.
contact email	zhouqian@omicsspace.com
dataset submitter

iProX dataset URI