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PXD012931

PXD012931 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted Mass Spectrometry of Brain and Pancreatic tissues
DescriptionQPrESTs are heavy labelled recombinant protein fragments covering regions of proteins with low homology and span over 50-150 amino acids of the sequence. They were produced as a part of Human Protein Atlas project in auxotrophic Escherichia coli and individually purified, serving as an excellent resource for MS-based protein quantification. In this project, a workflow for sample preparation of brain and pancreatic tissues with use of QPrESTs was developed together with a panel of parallel reaction monitoring (PRM) methods for quantification of more than 50 proteins using targeted mass spectrometry. In total, methods for quantification of 125 and 126 peptides in cortex and pancreas respectively were established. The technical robustness of the workflow was investigated on three technical replicates of pancreatic and three biological replicates of brain tissues. The results are reported in fmol/g of protein content in a tissue lysate and span with dynamic range over 5-6 orders of magnitude. The combination of QPrEST standards being added before digestion and state of the art mass spectrometry performing in PRM mode showed high degree of reproducibility where 78.8 % and 81.1 % of quantified proteins had coefficient of variation (CV) below 20 % in Cortex and Pancreas respectively.
HostingRepositoryPanoramaPublic
AnnounceDate2019-03-01
AnnouncementXMLSubmission_2019-03-01_14:12:50.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Kotol
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-01 14:07:54ID requested
12019-03-01 14:12:50announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Targeted Mass Spectrometry, QPrEST, PRM, Brain, Pancreas, CZI
Contact List
Bjorn Forsstrom
contact affiliationScience for Life Laboratory, Stockholm, Sweden
contact emailbjorn.forsstrom@scilifelab.se
lab head
David Kotol
contact affiliationScience for Life Laboratory, Stockholm, Sweden
contact emaildavid.kotol@scilifelab.se
dataset submitter
Full Dataset Link List
Panorama Public dataset URI