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PXD012920

PXD012920 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometric analysis of accumulated TDP-43 in amyotrophic lateral sclerosis brains
DescriptionTDP-43 is the major disease-associated protein involved in the pathogenesis and progression of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin-positive inclusions linked to TDP-43 pathology (FTLD-TDP). Abnormal phosphorylation, truncation and cytoplasmic mis-localization are known to be the characteristics for the aggregated forms of TDP-43, and gain of toxic abnormal TDP-43 or loss of function of physiological TDP-43 have been suggested as the cause of neurodegeneration. However, most of the post-translational modifications or truncation sites in the abnormal TDP-43 in brains of patients remain to be identified by protein chemical analysis. In this study, we carried out a highly sensitive liquid chromatography-mass spectrometry analysis of Sarkosyl-insoluble pathological TDP-43 from brains of ALS patients and identified several novel phosphorylation sites, deamidation sites, and cleavage sites. Almost all modifications were localized in the Gly-rich C-terminal half. Most of the cleavage sites identified in this study are novel and are located in N-terminal half, suggesting that these sites may be more accessible to proteolytic enzymes. The data obtained in this study provide a foundation for the molecular mechanisms of TDP-43 aggregation and ALS pathogenesis.
HostingRepositoryjPOST
AnnounceDate2020-03-01
AnnouncementXMLSubmission_2022-09-18_02:19:17.090.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFuyuki Kametani
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListunknown modification; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-28 23:20:40ID requested
12020-02-29 07:00:07announced
22020-03-19 00:45:06announced2020-03-19: Updated PubMed.
32021-02-04 16:39:49announced2021-02-04: Updated FTP location.
42022-09-18 02:19:17announced2022-09-18: Updated FTP location.
Publication List
Kametani F, Obi T, Shishido T, Akatsu H, Murayama S, Saito Y, Yoshida M, Hasegawa M, Mass spectrometric analysis of accumulated TDP-43 in amyotrophic lateral sclerosis brains. Sci Rep, 6():23281(2016) [pubmed]
Keyword List
submitter keyword: amyloid, TDP-43, inclusion body, phosphorylation, frontotemporal lobar degeneration with ubiquitin-positive inclusions, amyotrophic lateral sclerosis, proteomics, mass spectrometry
Contact List
Fuyuki Kametani
lab head
Fuyuki Kametani
contact affiliationTokyo Metropolitan Institute of Medical Science
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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