PXD012907

PXD012907 is an original dataset announced via ProteomeXchange.

Title	Alterations of redox and iron metabolism accompany development of HIV latency
Description	Metabolic alterations, such as oxidative stress, are hallmarks of HIV-1 infection. However, their influenceon the development of viral latency, and thus on HIV-1 persistence during antiretroviral therapy (ART),have just begun to be explored. We analyzed omics profiles ofin-vitroandin-vivomodels of infection byHIV-1 and its simian homolog SIVmac. We found that cells survive retroviral replication by upregulatingantioxidant pathways and intertwined iron import pathways. These changes are associated withremodeling of the redox sensitive promyelocytic leukemia protein nuclear bodies (PML NBs), an importantconstituent of nuclear architecture and a marker of HIV-1 latency. We found that PML is depleted inproductively infected cells and restored by ART. Moreover, we identified intracellular iron as a key linkbetween oxidative stress and PML depletion, thus supporting iron metabolism modulators aspharmacological tools to impair latency establishment.
HostingRepository	PRIDE
AnnounceDate	2020-03-03
AnnouncementXML	Submission_2020-05-27_04:36:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-28 04:59:02	ID requested
1	2020-03-03 00:15:05	announced
⏵ 2	2020-05-27 04:36:30	announced	2020-05-27: Updated publication reference for PubMed record(s): 32157726.

Shytaj IL, Lucic B, Forcato M, Penzo C, Billingsley J, Laketa V, Bosinger S, Stanic M, Gregoretti F, Antonelli L, Oliva G, Frese CK, Trifunovic A, Galy B, Eibl C, Silvestri G, Bicciato S, Savarino A, Lusic M, Alterations of redox and iron metabolism accompany the development of HIV latency. EMBO J, 39(9):e102209(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: oxidative stress

HIV-1 latency

SIVmac

Nrf2

iron

transferrin receptor-1

promyelocyticleukemia protein

nuclear bodies

Marina Lusic
contact affiliation	Center for Integrative Infectious Diseases (CIID) Integrative Virology Heidelberg University Clinic Im Neuenheimer Feld 344 69120 Heidelberg
contact email	marina.lusic@med.uni-heidelberg.de
lab head
Christian Frese
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	frese@mpiib-berlin.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012907

PRIDE project URI

• PRIDE
  1. PXD012907
     1. Label: PRIDE project
     2. Name: Alterations of redox and iron metabolism accompany development of HIV latency