PXD012903 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of protein regulation in cucumber leaves after Corynespora cassiicola inoculation: a proteomic approach |
Description | Corynespora leaf spot (CSL), caused by Corynespora cassiicola, has become one of the most important foliar diseases of cultivated cucumber. However, the defense mechanisms of cucumber plants in response to C. cassiicola are still poorly understood. Here, proteins from resistant plants were analyzed using isobaric tags for relative and absolute quantification (iTRAQ). A total of 286 differentially expressed proteins were identified (P<0.05, ratio>1.2 or <0.83) at 6 and 24 h after pathogen inoculation in the resistant cucumber cultivar Jinyou 38. Some of the early responses to C. cassiicola infection were revealed, and four vital clues regarding the resistance of Cucumis sativus to cucumber CLS were discovered. First, the proteomic approach revealed the modulation of signaling pathways in resistant cucumber plants in response to C. cassiicola infection. Second, the plant immune system recognizes the pathogen and initiates the expression of basal immune response proteins, including those related to defense and stress responses, signal transduction, cell metabolism and redox regulation. Third, the common stress pathways were activated by C. cassiicola; in particular, mildew resistance locus O (MLO) proteins played a crucial role in the prevention of CLS. Fourth, the rapid activation of the carbohydrate and secondary metabolic pathways, the modification and reinforcement of cell walls, and the adjustment of the apoplectic environment to high-stress conditions were crucial in cucumber resistance to CLS disease. Overall, our data increase the knowledge of incompatible interactions between plants and pathogens and provide new insight into the contribution of molecular processes in cucumber to disease resistance. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-26 |
AnnouncementXML | Submission_2019-09-26_06:51:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guangchao Y |
SpeciesList | scientific name: Cucumis sativus; NCBI TaxID: 3659; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-28 01:19:12 | ID requested | |
⏵ 1 | 2019-09-26 06:51:47 | announced | |
Publication List
Yu G, Yu Y, Fan H, Zhang D, Cui N, Wang X, Jia S, Yang Y, Zhao J, Analysis of Protein Synthesis in Cucumber Leaves after Inoculation with Corynespora cassiicola: A Proteomic Approach. Biochemistry (Mosc), 84(8):963-977(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Cucumber, Corynespora cassiicola, iTRAQ, Defense-related proteins, qRT-PCR, Histochemical staining |
Contact List
Haiyan Fan |
contact affiliation | College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China |
contact email | 1997500018@syau.edu.cn |
lab head | |
Guangchao Y |
contact affiliation | 15840135256 |
contact email | yugc7674@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012903
- Label: PRIDE project
- Name: Analysis of protein regulation in cucumber leaves after Corynespora cassiicola inoculation: a proteomic approach