PXD012895

PXD012895 is an original dataset announced via ProteomeXchange.

Title	Secreted parasite Pin1 isomerase stabilizes host PKM2 to reprogram host cell metabolism
Description	Metabolic reprogramming is an important feature of host-pathogen interactions and a hallmark of tumorigenesis. The intracellular apicomplexa parasite Theileria induces a Warburg-like effect in host leukocytes by hijacking signaling machineries, epigenetic regulators and transcriptional programs to create a transformed cell state. The molecular mechanisms underlying host cell transformation are unknown. Here we show that a parasite-encoded prolyl-isomerase, TaPin1, stabilizes host pyruvate kinase isoform M2 (PKM2) leading to HIF-1α-dependent regulation of metabolic enzymes, glucose uptake and transformed phenotypes in parasite-infected cells. Our results provide a direct molecular link between the secreted parasite TaPin1 protein and host gene expression programs. This study demonstrates the importance of prolyl isomerization in the parasite manipulation of host metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:59.065.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justine Marsolier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-27 07:05:30	ID requested
1	2019-11-12 18:58:05	announced
⏵ 2	2024-10-22 04:54:05	announced	2024-10-22: Updated project metadata.

10.1038/s42003-019-0386-6;

Marsolier J, Perichon M, Weitzman JB, Medjkane S, Secreted parasite Pin1 isomerase stabilizes host PKM2 to reprogram host cell metabolism. Commun Biol, 2():152(2019) [pubmed]

submitter keyword: LS-MS/MS,Pin1, PKM

Jonathan WEITZMAN
contact affiliation	UMR7216 Paris France
contact email	jonathan.weitzman@univ-paris-diderot.fr
lab head
Justine Marsolier
contact affiliation	UMR7216 Paris France
contact email	marsolier.justine@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012895

PRIDE project URI

• PRIDE
  1. PXD012895
     1. Label: PRIDE project
     2. Name: Secreted parasite Pin1 isomerase stabilizes host PKM2 to reprogram host cell metabolism