PXD012889 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Distinct protein signature of hypertension-induced damage in the renal proteome of the two-kidney, one-clip ratmodel |
Description | Background: Hypertensive nephrosclerosis is one of the most frequent causes of chronic kidney failure. Proteome analysis potentially improves the pathophysiological understanding and diagnostic precision of this disorder. In the present exploratory study, we investigated experimental nephrosclerosis in the two-kidney, one-clip (2K1C) hypertensive rat model. Methods: The renal cortex proteome from juxtamedullary cortex and outer cortex of 2K1C male Wistar–Hannover rats (n¼4) was compared with the sham-operated controls (n¼6), using mass spectrometry-based quantitative proteomics. We combined a high abundant plasma protein depletion strategy with an extended liquid chromatographic gradient to improve peptide and protein identification. Immunohistology was used for independent confirmation of abundance. Results: We identified 1724 proteins, of which 1434 were quantified with at least two unique peptides. Comparative proteomics revealed 608 proteins, including the platelet-derived growth factor receptor-b signalling pathway, with different abundances between the non-clipped kidney of hypertensive 2K1C rats and the corresponding kidney of the normotensive controls (P<0.05, absolute fold change 1.5). Among the most significantly altered proteins in the whole cortex were periostin, transgelin, and creatine kinase B-type. Relative abundance of periostin alone allowed clear classification of 2K1C and controls. Enrichment of periostin in 2K1C rats was verified by immunohistology, showing positivity especially around the fibrotic vessels. Conclusion: The proteome is altered in hypertensioninduced kidney damage. We propose periostin, especially in combination with transgelin and creatine kinase B-type, as possible proteomic classifier to distinguish hypertensive nephrosclerosis from the normal tissue. This classifier needs to be further validated with respect to early diagnosis of fibrosis, prognosis, and its potential as a novel molecular target for pharmacological interventions. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-02 |
AnnouncementXML | Submission_2019-03-02_00:30:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kenneth Finne |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-27 06:24:59 | ID requested | |
1 | 2019-03-01 07:31:06 | announced | |
⏵ 2 | 2019-03-02 00:30:18 | announced | Updated project metadata. |
Publication List
Vethe H, Finne K, Skogstrand T, Vaudel M, Vikse BE, Hultstr, ö, m M, Placier S, Scherer A, Tenstad O, Marti HP, Distinct protein signature of hypertension-induced damage in the renal proteome of the two-kidney, one-clip rat model. J Hypertens, 33(1):126-35(2015) [pubmed] |
Keyword List
submitter keyword: Kidney, Hypertension, 2K1C, Juxtamedullary cortex |
Contact List
Hans-Peter Marti |
contact affiliation | Department of Clinical Medicine, University of Bergen, Norway |
contact email | hans-peter.marti@uib.no |
lab head | |
Kenneth Finne |
contact affiliation | University of Bergen |
contact email | kenneth.finne@uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012889
- Label: PRIDE project
- Name: Distinct protein signature of hypertension-induced damage in the renal proteome of the two-kidney, one-clip ratmodel