PXD012888

PXD012888 is an original dataset announced via ProteomeXchange.

Title	Single shot CZE-MS/MS for phosphoproteomics
Description	The dependence of capillary zone electrophoresis separations on the charge state of analyte is useful for the analysis of many post-translational modifications in proteins. In this work, we coupled CZE to an Orbitrap Fusion Lumos Tribrid platform with advanced peak determination algorithm for phosphoproteomics analysis. A linear polyacrylamide coated capillary with very low electroosmotic flow was used for the separation. The optimal injection volume is between 100 nL and 150 nL of phosphopeptides dissolved in 30 mM ammonium bicarbonate (pH 8.2) buffer, which produces a dynamic pH junction sample injection. Larger injection volumes resulted in serious peak broadening and decreased numbers of phosphopeptide identifications. The optimized system identified 4405 phosphopeptides from 220 ng of enriched phosphopeptides from mouse brain, which represents the state-of-the-art result for single shot CZE-ESI-MS/MS based phosphoproteome analysis.
HostingRepository	PRIDE
AnnounceDate	2019-06-13
AnnouncementXML	Submission_2019-06-13_01:30:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenbin Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-27 06:24:54	ID requested
⏵ 1	2019-06-13 01:30:42	announced

Zhang Z, Hebert AS, Westphall MS, Coon JJ, Dovichi NJ, Single-Shot Capillary Zone Electrophoresis-Tandem Mass Spectrometry Produces over 4400 Phosphopeptide Identifications from a 220 ng Sample. J Proteome Res, 18(8):3166-3173(2019) [pubmed]

submitter keyword: Phosphoproteomics, CZE, Orbitrap Fusion Lumos Tribrid, Advanced Peak Determination Algorithm

Zhenbin Zhang
contact affiliation	University of Notre Dame
contact email	zzhang9@nd.edu
lab head
Zhenbin Zhang
contact affiliation	University of Notre Dame
contact email	zzhang9@nd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012888

PRIDE project URI

• PRIDE
  1. PXD012888
     1. Label: PRIDE project
     2. Name: Single shot CZE-MS/MS for phosphoproteomics