PXD012886

PXD012886 is an original dataset announced via ProteomeXchange.

Title	The dual prey-inactivation strategy of spiders - In-depth venomic analysis of Cupiennius salei
Description	Most knowledge on spider venoms concerns neurotoxins acting on ion channels, whereas proteins and their significance for the envenomation process are neglected. The comprehensive analysis presented here of the venom gland transcriptome and proteome of Cupiennius salei with a focus on proteins and cysteine-containing peptides offers new insight into the structure and function of spider venom, presented here as dual prey-inactivation strategy. After venom injection, many enzymes and proteins, dominated by α-amylase, angiotensin-converting enzyme, and cysteine-rich secretory proteins, interact with main metabolic pathways, leading to major disturbance of the cellular homeostasis. Hyaluronidase and cytolytic peptides destroy tissue and membranes, thus supporting the spread of other venom compounds. We detected 81 transcripts of neurotoxins from 13 peptide families, whereof two families comprise 93.7% of all cysteine-containing peptides. This raises the question of the importance of the other low-expressed peptide families. The identification of a venom gland-specific defensin-like peptide and an aga-toxin-like peptide in the hemocytes offers an important clue on the recruitment and neofunctionalization of body proteins and peptides as the origin of toxins.
HostingRepository	PRIDE
AnnounceDate	2019-03-25
AnnouncementXML	Submission_2019-03-25_06:22:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Langenegger
SpeciesList	scientific name: Cupiennius salei (American wandering spider); NCBI TaxID: 6928;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-27 00:08:58	ID requested
⏵ 1	2019-03-25 06:22:55	announced

Kuhn-Nentwig L, Langenegger N, Heller M, Koua D, Nentwig W, . Toxins (Basel), 11(3):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Cupiennius salei spider venom

Lucia Kuhn-Nentwig
contact affiliation	Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland
contact email	lucia.kuhn@iee.unibe.ch
lab head
Nicolas Langenegger
contact affiliation	Institute of Ecology and Evolution, University of Bern, Switzerland
contact email	nicolas.langenegger@iee.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012886

PRIDE project URI

• PRIDE
  1. PXD012886
     1. Label: PRIDE project
     2. Name: The dual prey-inactivation strategy of spiders - In-depth venomic analysis of Cupiennius salei