PXD012882

PXD012882 is an original dataset announced via ProteomeXchange.

Title	Direct Nucleosome Binding of Borealin Secures Chromosome Association of the Chromosomal Passenger Complex
Description	CPC-NCP complexes were crosslinked using EDC and analysed by mass spectrometry. The results showed Borealin is critical for nucleosome binding made extensive contacts with NCPs, whereas Survivin interactions are mostly limited to the BIR domain and the H3 N-terminal tail as expected. Mapping the crosslinks onto the three dimensional structures of NCP and CPC suggests a model where the localisation module of the CPC together with a highly basic N-terminal tail of Borealin docks onto the acidic patch formed by H2A and H2B, a surface commonly involved in nucleosome recognition. This interaction may orient the Survivin BIR domain to facilitate binding of histone H3 tail phosphorylated at Thr3. Notably, Borealin loop residues trace along the DNA-histone interface implying its major contribution to nucleosome binding involves both protein-histone and possibly protein-DNA contacts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:54:03.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-26 07:48:14	ID requested
1	2019-09-30 02:29:15	announced
2	2019-10-11 00:05:42	announced	2019-10-11: Updated publication reference for PubMed record(s): 31570499.
⏵ 3	2024-10-22 04:54:06	announced	2024-10-22: Updated project metadata.

10.1083/jcb.201905040;

Abad MA, Ruppert JG, Buzuk L, Wear M, Zou J, Webb KM, Kelly DA, Voigt P, Rappsilber J, Earnshaw WC, Jeyaprakash AA, Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex. J Cell Biol, 218(12):3912-3925(2019) [pubmed]

curator keyword: Biological

submitter keyword: Nucleosome core particles,Chromosomal Passenger Complex, Crosslinking

Juri Rappsilber
contact affiliation	1. Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK 2. Technical University, Berlin 13355, Germany
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD012882

PRIDE project URI

• PRIDE
  1. PXD012882
     1. Label: PRIDE project
     2. Name: Direct Nucleosome Binding of Borealin Secures Chromosome Association of the Chromosomal Passenger Complex