PXD012881 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The dual prey-inactivation strategy of spiders - In-depth venomic analysis of Cupiennius salei |
Description | Most knowledge on spider venoms concerns neurotoxins acting on ion channels, whereas proteins and their significance for the envenomation process are neglected. The comprehensive analysis presented here of the venom gland transcriptome and proteome of Cupiennius salei with a focus on proteins and cysteine-containing peptides offers new insight into the structure and function of spider venom, presented here as dual prey-inactivation strategy. After venom injection, many enzymes and proteins, dominated by α-amylase, angiotensin-converting enzyme, and cysteine-rich secretory proteins, interact with main metabolic pathways, leading to major disturbance of the cellular homeostasis. Hyaluronidase and cytolytic peptides destroy tissue and membranes, thus supporting the spread of other venom compounds. We detected 81 transcripts of neurotoxins from 13 peptide families, whereof two families comprise 93.7% of all cysteine-containing peptides. This raises the question of the importance of the other low-expressed peptide families. The identification of a venom gland-specific defensin-like peptide and an aga-toxin-like peptide in the hemocytes offers an important clue on the recruitment and neofunctionalization of body proteins and peptides as the origin of toxins. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-25 |
AnnouncementXML | Submission_2019-03-25_06:50:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nicolas Langenegger |
SpeciesList | scientific name: Cupiennius salei (American wandering spider); NCBI TaxID: 6928; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-26 05:01:49 | ID requested | |
⏵ 1 | 2019-03-25 06:50:50 | announced | |
Publication List
Kuhn-Nentwig L, Langenegger N, Heller M, Koua D, Nentwig W, . Toxins (Basel), 11(3):(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Cupiennius salei spider venom |
Contact List
Lucia Kuhn-Nentwig |
contact affiliation | Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland |
contact email | lucia.kuhn@iee.unibe.ch |
lab head | |
Nicolas Langenegger |
contact affiliation | Institute of Ecology and Evolution, University of Bern, Switzerland |
contact email | nicolas.langenegger@iee.unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012881 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012881
- Label: PRIDE project
- Name: The dual prey-inactivation strategy of spiders - In-depth venomic analysis of Cupiennius salei