PXD012879

PXD012879 is an original dataset announced via ProteomeXchange.

Title	MSCs OA SILAC Mass spectrometry imaging
Description	In osteoarthritis (OA), impairment of cartilage regeneration can be related to a defective chondrogenic differentiation of mesenchymal stromal cells (MSCs). Therefore, understanding the proteomic- and metabolomic-associated molecular events during the chondrogenesis of MSCs could provide alternative targets for therapeutic intervention. Here, a SILAC-based proteomic analysis identified 43 proteins related with metabolic pathways whose abundance was significantly altered during the chondrogenesis of OA human bone marrow MSCs (hBMSCs). Then, the level and distribution of metabolites was analyzed in these cells and healthy controls by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), leading to the recognition of characteristic metabolomic profiles at the early stages of differentiation. Finally, integrative pathway analysis showed that UDP-glucuronic acid synthesis and amino sugar metabolism were downregulated in OA hBMSCs during chondrogenesis compared to healthy cells. Alterations in these metabolic pathways may disturb the production of hyaluronic acid (HA) and other relevant cartilage extracellular matrix (ECM) components. This work provides a novel integrative insight into the molecular alterations of osteoarthritic MSCs and potential therapeutic targets for OA drug development through the enhancement of chondrogenesis.
HostingRepository	PRIDE
AnnounceDate	2020-01-27
AnnouncementXML	Submission_2020-01-29_05:47:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Beatriz Rocha
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	4800 Proteomics Analyzer; solariX

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-26 01:44:49	ID requested
1	2020-01-27 00:52:04	announced
⏵ 2	2020-01-29 05:47:02	announced	2020-01-29: Updated publication reference for PubMed record(s): 31980557.

Rocha B, Cillero-Pastor B, Eijkel G, Calamia V, Fernandez-Puente P, Paine MRL, Ruiz-Romero C, Heeren RMA, Blanco FJ, Integrative Metabolic Pathway Analysis Reveals Novel Therapeutic Targets in Osteoarthritis. Mol Cell Proteomics, 19(4):574-588(2020) [pubmed]

submitter keyword: mass spectrometry, proteins, metabolites, imaging, ostearthritis

Francisco Javier Blanco García
contact affiliation	Grupo de Investigación de Reumatología, INIBIC-Complejo Hospitalario Universitario de A Coruña, SERGAS, Departamento de Medicina Universidad de A Coruña, A Coruña, Spain
contact email	fblagar@sergas.es
lab head
Beatriz Rocha
contact affiliation	Proteomics Group-ProteoRed/ISCIII, Grupo de Investigación de Reumatología (GIR), INIBIC – Hospital Universitario de A Coruña, A Coruña, Spain
contact email	beatriz.rocha.loureda@sergas.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD012879

PRIDE project URI

• PRIDE
  1. PXD012879
     1. Label: PRIDE project
     2. Name: MSCs OA SILAC Mass spectrometry imaging