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PXD012874

PXD012874 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of different human CD8 T cell subsets by superior cytotoxicity in human T cells
DescriptionCytotoxic CD8+ T cells can effectively kill target cells by producing cytokines, chemokines and granzymes. Expression of these effector molecules is however highly divergent, and tools that identify and pre-select potent killer cells are lacking. Human CD8+ T cells can be divided into IFNGand IL-2 producing cells. Unbiased RNA-sequencing and proteomics analysis on cytokine-producing fixed CD8+ T cells revealed that IL-2+ T cells produce helper cytokines, and that IFNG+ T cells produce cytotoxic molecules. IFNG+ cytotoxic T cells expressed the surface marker CD29 already prior to stimulation. CD29 also marked T cells with cytotoxic gene expression from different tissues in single-cell RNA-sequencing data. Notably, the cytotoxic features of CD29+ T cells were maintained during cell culture, suggesting a stable phenotype. Pre-selecting CD29-expressing MART1 TCR-engineered T cells potentiated the killing of target cells. We therefore propose that selecting for CD29+ T cells could boost the anti-tumoral activity of T cell therapeutics.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:25:47.641.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaartje van den Biggelaar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-25 08:48:08ID requested
12021-09-08 09:06:06announced
22024-10-22 05:25:56announced2024-10-22: Updated project metadata.
Publication List
10.1073/pnas.1913940117;
Nicolet BP, Guislain A, van Alphen FPJ, Gomez-Eerland R, Schumacher TNM, van den Biggelaar M, Wolkers MC, T cells with an increased cytotoxic potential. Proc Natl Acad Sci U S A, 117(12):6686-6696(2020) [pubmed]
Keyword List
submitter keyword: Human, IL2, IFNG, CD8, T cells
Contact List
Maartje van den Biggelaar
contact affiliationLaboratory of Proteomics, Department of Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam, the Netherlands
contact emailm.vandenbiggelaar@sanquin.nl
lab head
Maartje van den Biggelaar
contact affiliationSanquin Research
contact emailm.vandenbiggelaar@sanquin.nl
dataset submitter
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Dataset FTP location
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