PXD012872

PXD012872 is an original dataset announced via ProteomeXchange.

Title	Detecting single amino acids and small peptides by combining isobaric tags and peptidomics
Description	Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment we found that by combining isobaric tags and peptidomics, and by targeting singly charged molecules, we were able to identify a significant amount of single amino acids and small endogenous peptides using a basic mass-based identification approach. While there is still room for improvement, our simple test indicates that a limited amount of extra work when setting up the mass spectrometry experiment could potentially lead to a wealth of additional information.
HostingRepository	PRIDE
AnnounceDate	2019-06-26
AnnouncementXML	Submission_2019-06-26_02:14:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ragnhild Reehorst Lereim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 07:31:10	ID requested
⏵ 1	2019-06-26 02:14:46	announced

Burger B, Lereim RR, Berven FS, Barsnes H, Detecting single amino acids and small peptides by combining isobaric tags and peptidomics. Eur J Mass Spectrom (Chichester), 25(6):451-456(2019) [pubmed]

submitter keyword: Peptidomics, Cerebrospinal fluid, TMT, tandem mass tag, isobaric peptidomics

Harald Barsnes
contact affiliation	Computational Biology Unit (CBU), Department of Informatics, University of Bergen, Bergen, Norway Proteomics Unit (PROBE), Department of Biomedicine, University of Bergen, Bergen, Norway
contact email	Harald.Barsnes@uib.no
lab head
Ragnhild Reehorst Lereim
contact affiliation	Proteomics unit at the University of Bergen (PROBE)
contact email	rle041@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012872

PRIDE project URI

• PRIDE
  1. PXD012872
     1. Label: PRIDE project
     2. Name: Detecting single amino acids and small peptides by combining isobaric tags and peptidomics