PXD012871

PXD012871 is an original dataset announced via ProteomeXchange.

Title	Identification of a USP9X Substrate NFX1-123 by SILAC-based Quantitative Proteomics
Description	The deubiquitinase USP9X related with multiple diseases including neurodegeneration, epilepsy and various type of tumor, by targeting different substrates. In our study, to deep study USP9X functional candidate substrates, we performed SILAC-based quantitative proteomic to compare knockdown USP9X with wild type HeLa cells to screen USP9X potential substrates. Further, we carried out Flag-NFX1-123 affinity tag-based interaction mass spectrometry method and verified that the X-box binding nuclear factor NFX1-123 is a substrate of USP9X. Further experimental evidences confirmed that USP9X stabilizedNFX1-123 protein level rather than mRNA levels via direct interaction and efficient deubiquitination of NFX1-123 protein
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:56.426.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiangling chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 07:29:03	ID requested
1	2019-05-13 00:40:29	announced
⏵ 2	2024-10-22 04:54:04	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00139;

Chen X, Lu D, Gao J, Zhu H, Zhou Y, Gao D, Zhou H, Identification of a USP9X Substrate NFX1-123 by SILAC-Based Quantitative Proteomics. J Proteome Res, 18(6):2654-2665(2019) [pubmed]

submitter keyword: USP9X;NFX1-123;SILAC

Hu Zhou
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
contact email	zhouhu@simm.ac.cn
lab head
xiangling chen
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	xianglingchen@simm.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012871

PRIDE project URI

• PRIDE
  1. PXD012871
     1. Label: PRIDE project
     2. Name: Identification of a USP9X Substrate NFX1-123 by SILAC-based Quantitative Proteomics