PXD012867

PXD012867 is an original dataset announced via ProteomeXchange.

Title	The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance
Description	Misfolded proteins in the endoplasmic reticulum (ER) activate the unfolded protein response (UPR), which enhances protein folding to restore homeostasis. Additional pathways respond to ER stress, but how they help counteract protein misfolding is incompletely understood. Here, we develop a titratable system for the induction of ER stress in yeast to enable a genetic screen for factors that augment stress resistance independently of the UPR. We identify the proteasome biogenesis regulator Rpn4 and show that it cooperates with the UPR. Rpn4 abundance increases during ER stress, first by a post-transcriptional, then by a transcriptional mechanism. Induction of RPN4 transcription is triggered by cytosolic mislocalization of secretory proteins, is mediated by multiple signaling pathways and accelerates clearance of misfolded proteins from the cytosol. Thus, Rpn4 and the UPR are complementary elements of a modular cross-compartment response to ER stress.
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-05-27_00:08:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Borner
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 05:21:26	ID requested
1	2019-03-13 02:28:19	announced
⏵ 2	2019-05-27 00:08:26	announced	Updated publication reference for PubMed record(s): 30865586.

Schmidt RM, Schessner JP, Borner GH, Schuck S, The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance. Elife, 8():(2019) [pubmed]

curator keyword: Biological

submitter keyword: AP-4, autophagy, vesicles, human cells, Dynamic Organellar Maps

Georg H H Borner
contact affiliation	Max Planck Institute of Biochemistry Department of Proteomics and Signal Transduction Am Klopferspitz 18 82152 Martinsried, Germany
contact email	borner@biochem.mpg.de
lab head
Georg Borner
contact affiliation	Max Planck Institute of Biochemistry
contact email	borner@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012867
     1. Label: PRIDE project
     2. Name: The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance