PXD012862

PXD012862 is an original dataset announced via ProteomeXchange.

Title	Role of iron in the regulation of EMT
Description	The biological process termed Epithelial-to-Mesenchymal Transition (EMT) plays a central role in cancer cell invasion, metastasis, self-renewal and resistance to therapy(1,2). Here, using western blot technique, we show that H3K9me2 decreases when MDA-MB-468 breast cancer cells undergo EMT upon EGF. We validate this decrease by performing high-resolution MS/MS spectrum. Interestingly, we find that H3K9me2 is associated with mesenchymal genes regulation. 1. Nieto, M. A., Huang, R. Y., Jackson, R. A. & Thiery, J. P. EMT: 2016. Cell 166, 21–45 (2016). 2. Puisieux, A. & Brabletz, T. & Caramel, J. Oncogenic roles of EMT-inducing transcription factors. Nat. Cell Biol. 16, 488–494 (2014).
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2019-09-25_06:57:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Arras
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	trimethylated residue; acetylated residue; dimethylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 03:32:13	ID requested
⏵ 1	2019-09-25 06:57:45	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Breast cancer, EMT, histones, MS/MS spectrum

Damarys Loew
contact affiliation	Laboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University
contact email	Damarys.Loew@curie.fr
lab head
Guillaume Arras
contact affiliation	Institut Curie
contact email	guillaume.arras@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD012862

PRIDE project URI

• PRIDE
  1. PXD012862
     1. Label: PRIDE project
     2. Name: Role of iron in the regulation of EMT