PXD012854

PXD012854 is an original dataset announced via ProteomeXchange.

Title	Regulation of Borrelia burgdorferi-induced macrophage activity by spirochetal-regulated surface receptors
Description	Macrophages mediate the elimination of pathogens by phagocytosis resulting in the activation of specific signaling pathways that lead to the production of cytokines, chemokines and other factors. Borrelia burgdorferi, the causative agent of Lyme disease, causes a wide variety of pro-inflammatory symptoms. The proinflammatory capacity of macrophages is intimately related to the internalization of the spirochete. However, most receptors mediating this process are largely unknown. We have applyedapplied a multiomic approach, including the proteomic analysis of B. burgdorferi-containing phagosome-enriched fractions, to identify surface receptors that are involved in the phagocytic capacity of macrophages as well as their inflammatory output. Sucrose gradient protein fractions of human monocyte-derived macrophages exposed to B. burgdorferi contained the phagocytic receptor, CR3/CD14 highlighting the major role played by these proteins in spirochetal phagocytosis. Among others, Other proteins identified proteins include C-type lectins, scavenger receptors or siglecs, and contain uPAR and MARCO. We also identified the Fc gamma receptor pathway as involved both in the phagocytosis of , and TNF induction by B. burgdorferi in the absence of antibodies. The common gamma chain, FcR, mediates the phagocytosis of the spirochete, likely through Fc receptors and C-type lectins, in a process that involves Syk activation. Overall, these findings highlight the complex array of receptors involved in the phagocytic response of macrophages to B. burgdorferi.
HostingRepository	PRIDE
AnnounceDate	2019-12-06
AnnouncementXML	Submission_2019-12-06_06:50:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Elortza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 01:19:44	ID requested
⏵ 1	2019-12-06 06:50:12	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Phagosome, Proteomics, Lyme disease

Juan Anguita
contact affiliation	Inflammation and Macrophage Plasticity Lab, CIC bioGUNE
contact email	janguita@cicbiogune.es
lab head
Felix Elortza
contact affiliation	Proteomics Platform
contact email	felortza@cicbiogune.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012854
     1. Label: PRIDE project
     2. Name: Regulation of Borrelia burgdorferi-induced macrophage activity by spirochetal-regulated surface receptors