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PXD012853

PXD012853 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEpigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription
DescriptionSperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional program for embryonic development. We show for the first time that remodelling of histones during spermiogenesis results in the retention of methylated histone H3 at the same genomic location in every sperm cell. This homogeneously methylated fraction of histone H3 in the sperm genome is maintained during early embryonic replication. Such methylated histone fraction resisting postfertilisation reprogramming marks developmental genes whose expression is perturbed upon experimental reduction of histone methylation. A similar homogeneously methylated histone H3 fraction is detected in human sperm. Altogether, we uncover a conserved mechanism of paternal epigenetic information transmission to the embryo through the homogeneous retention of methylated histone in a sperm cells population.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:06:24.417.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterjerome jullien
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue; deamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-25 01:19:27ID requested
12020-05-28 23:42:21announced
22020-07-18 18:40:16announced2020-07-19: Updated publication reference for PubMed record(s): 32661239.
32024-10-22 05:06:25announced2024-10-22: Updated project metadata.
Publication List
Oikawa M, Simeone A, Hormanseder E, Teperek M, Gaggioli V, O'Doherty A, Falk E, Sporniak M, D'Santos C, Franklin VNR, Kishore K, Bradshaw CR, Keane D, Freour T, David L, Grzybowski AT, Ruthenburg AJ, Gurdon J, Jullien J, Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription. Nat Commun, 11(1):3491(2020) [pubmed]
10.1038/s41467-020-17238-w;
Keyword List
curator keyword: Biological
submitter keyword: epigenetics, programming,sperm, ice-ChIP, LC-MS/MS
Contact List
jerome jullien
contact affiliationgurdon institute
contact emailj.jullien256@gmail.com
lab head
jerome jullien
contact affiliationgurdon institute
contact emailj.jullien256@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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