PXD012851

PXD012851 is an original dataset announced via ProteomeXchange.

Title	Endo-Lysosomal and Ubiquitin CSF Biomarker Discovery by solid-phase extraction and LC-MS/MS
Description	Dysfunctional proteostasis is increasingly implicated in neurodegenerative diseases which is exemplified by an accumulation of protein aggregates in the brain in e.g. Alzheimer's and Parkinson's disease. In addition, in Alzheimer's disease there is an accumulation of autophagic vesicles within neurites. Susceptibility in Parkinson's disease has convincingly been connected with genetic mutations associated with lysosomal storage disorders. Proteostasis is maintained in the cell by the endo-lysosomal system, autophagy and the ubiquitin-proteasome system. Identifying biomarkers reflecting proteostasis might increase our understanding of neurodegenerative disorders and serve as tools to monitor the effects of new treatment strategies. To date no treatment has proven effective, neither in Alzheimer’s disease nor Parkinson’s disease. The aim of this project was to identify proteins involved in the endo-lysosomal system, autophagy and the ubiquitin-proteasome system in CSF. This information will be used to select and target relevant proteins for quantitative analysis using e.g. PRM or SRM to evaluate these proteins as potential biomarkers in neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:39.379.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Sjödin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 01:19:03	ID requested
1	2019-09-25 06:37:56	announced
⏵ 2	2024-10-22 04:53:42	announced	2024-10-22: Updated project metadata.

Sj, ö, din S, Brinkmalm G, Ö, hrfelt A, Parnetti L, Paciotti S, Hansson O, Hardy J, Blennow K, Zetterberg H, Brinkmalm A, Endo-lysosomal proteins and ubiquitin CSF concentrations in Alzheimer's and Parkinson's disease. Alzheimers Res Ther, 11(1):82(2019) [pubmed]

10.1186/s13195-019-0533-9;

curator keyword: Biomedical

submitter keyword: Human, SPE, CSF, LC-MS/MS

Henrik Zetterberg
contact affiliation	Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Sahlgrenska Academy at the University of Gothenburg, Mölndal, Sweden Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Mölndal, Sweden Department of Molecular Neuroscience, University College London Institute of Neurology, Queen Square, London, UK UK Dementia Research Institute at UCL, London, United Kingdom
contact email	henrik.zetterberg@clinchem.gu.se
lab head
Simon Sjödin
contact affiliation	University of Gothenburg
contact email	simon.sjodin@neuro.gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD012851

PRIDE project URI

• PRIDE
  1. PXD012851
     1. Label: PRIDE project
     2. Name: Endo-Lysosomal and Ubiquitin CSF Biomarker Discovery by solid-phase extraction and LC-MS/MS