PXD012848

PXD012848 is an original dataset announced via ProteomeXchange.

Title	Isobaric tags for relative and absolute quantification based on quantitative proteomic analysis on inhibitor of apoptosis protein-like protein-2 knocked-down MCF-7 breast cancer cells
Description	Although the inhibitor of apoptosis protein-like protein-2 (ILP-2) is regarded as a novel growth enhancer for breast cancer, its mechanism on breast cancer cell growth remains elusive and requires further study. In this study, isobaric tags were applied for relative and absolute quantification (iTRAQ) analysis on two groups of MCF-7 (Michigan Cancer Foundation-7) breast cancer cells, namely, siRNA-5 group (knocked down, KD group) and negative control (NC) group, to analyse the protein expression profiles correlated to ILP-2 during breast cancer cell growth. Western blot was applied to verify the iTRAQ data. The results indicated that a total of 4065 proteins were identified in breast cancer cells, and 241 of these proteins are differentially expressed (fold change ≥ 1.20 or ≤ 0.83 and P<0.05). A total of 156 up- and 85 down-regulated proteins are found in siRNA-5 group versus NC group. These differentially expressed proteins are principally correlated to the ECM (extracellular matrix) -receptor interaction, and the proteins from the top 10 biological processes are correlated to signal transduction, regulation of cell proliferation and immune system processes. The expression of AGA (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase), MT1E (metallothionein-1E) and TDO2 (tryptophan 2,3-dioxygenase) increases when the protein expression of ILP-2 is knocked down. These results suggested that ILP-2 has an important role in breast cancer cell growth via protein interaction and provided comprehensive insights into biochemical pathways and regulation networks correlated to ILP-2.
HostingRepository	iProX
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2023-03-01_23:02:54.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MingJun Xiang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-24 23:30:35	ID requested
1	2021-12-28 19:12:00	announced
⏵ 2	2023-03-01 23:02:59	announced	2023-03-02: Update publication information.

Xiang S, Zhu L, Zhang Z, Wang S, Cui R, Xiang M, 2 on breast cancer cell proliferation. Mol Med Rep, 25(3):(2022) [pubmed]

submitter keyword: breast cancer, inhibitor of apoptosis protein-like protein-2, isobaric tags for relative and absolute quantification, proteome, MCF-7

MingJun Xiang
contact affiliation	Jishou University
contact email	xmj688@163.com
lab head
MingJun Xiang
contact affiliation	Jishou University
contact email	xmj688@163.com
dataset submitter

iProX dataset URI