PXD012842

PXD012842 is an original dataset announced via ProteomeXchange.

Title	Cell envelope adaptation and minimal Tat translocation are functionally intertwined
Description	Protein secretion is key for bacterial growth, survival and adaptation. In the Gram-positive bacterium Bacillus subtilis, the twin-arginine (Tat) pathway serves in the exclusive secretion of proteins with bound cofactors. The Bacillus Tat pathway stands out for its minimalist nature with two independently acting translocases being composed of dedicated TatA and TatC subunits only. Here we addressed the question whether one of these translocases, TatAyCy, recruits additional common cellular components for activity. To this end, TatAyCy was purified by affinity chromatography and gel filtration, and interacting co-purified proteins were identified by mass spectrometry. This revealed the cell envelope stress responsive LiaH protein as an appendix to the TatAyCy complex. Importantly, our functional studies show that Tat expression is tightly trailed by LiaH induction, and that LiaH itself determines the capacity and quality of Tat-dependent protein translocation. Altogether, our observations show that protein translocation by the minimal Tat translocase TatAyCy is tightly intertwined with an adequate bacterial response to cell envelope stress. This is consistent with a critical need to maintain cellular homeostasis, especially when the membrane is widely opened to permit passage of large fully-folded proteins via Tat.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:41.369.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minia Antelo
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis; NCBI TaxID: 135461;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 07:19:47	ID requested
1	2022-08-11 05:28:46	announced
⏵ 2	2024-10-22 05:38:42	announced	2024-10-22: Updated project metadata.

Bernal-Cabas M, Miethke M, Antelo-Varela M, Aguilar Su, á, rez R, Neef J, Sch, ö, n L, Gabarrini G, Otto A, Becher D, Wolf D, van Dijl JM, Functional association of the stress-responsive LiaH protein and the minimal TatAyCy protein translocase in Bacillus subtilis. Biochim Biophys Acta Mol Cell Res, 1867(8):118719(2020) [pubmed]

10.1016/j.bbamcr.2020.118719;

curator keyword: Biological

submitter keyword: Bacillus, LiaH, TatCy,Twin-arginine, TatAy, translocation, LiaRS

Jan Maarten van Dijl
contact affiliation	University of Groningen, University Medical Center Groningen, Department of Medical Microbiology, Hanzeplein 1, P.O. box 30001, 9700 RB, Groningen, The Netherlands
contact email	j.m.van.dijl01@umcg.nl
lab head
Minia Antelo
contact affiliation	Institute of Microbiology University of Greifswald
contact email	minia.antelovarela@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD012842

PRIDE project URI

• PRIDE
  1. PXD012842
     1. Label: PRIDE project
     2. Name: Cell envelope adaptation and minimal Tat translocation are functionally intertwined