PXD012837

PXD012837 is an original dataset announced via ProteomeXchange.

Title	IP-MS analysis .
Description	It has recently been shown that HSV-1 infection induces widespread defect in host gene transcription termination while viral genes are unaffected. However, The underlying mechanism remain poorly understood. According to our sequencing data, ICP27 as an immediate early protein of HSV-1 was involved in disrupting host transcription termination. To elucidate the underlying mechanism, we performed IP-MS to figure out interaction proteins with ICP27. Other than export proteins, 3’ end processing factors strongly associated with ICP27 based on this IP-MS data. Pre-mRNA 3’ end processing is required for transcription termination. As expected, this MS data as well as our in vitro and in vivo data suggested that ICP27 functions in pre-mRNA 3’ end processing to regulate transcription termination in a sequence dependent manner.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:24:09.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 03:42:30	ID requested
⏵ 1	2024-10-07 10:24:10	announced

Wang X, Hennig T, Whisnant AW, Erhard F, Prusty BK, Friedel CC, Forouzmand E, Hu W, Erber L, Chen Y, Sandri-Goldin RM, D, ö, lken L, Shi Y, Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27. Nat Commun, 11(1):293(2020) [pubmed]

10.1038/s41467-019-14109-x;

submitter keyword: ICP27, Immunoprecipitation,HSV, 3’ end processing

Yue Chen
contact affiliation	University of Minnesota at Twin Cities
contact email	yuechen@umn.edu
lab head
Yue Chen
contact affiliation	University of Minnesota
contact email	ychen2001@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD012837

PRIDE project URI

• PRIDE
  1. PXD012837
     1. Label: PRIDE project
     2. Name: IP-MS analysis