PXD012836

PXD012836 is an original dataset announced via ProteomeXchange.

Title	Approaching absolute quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29
Description	Protein extracts of three yeast strains (Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS6556 and Yarrowia lipolytica W29) cultivated in chemostats under different conditions. Representative samples containing aliquots of all conditions for each yeast strain were spiked with UPS2 standard (Sigma) to estimate absolute values in fmol. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: - Standard condition : 30°C, pH 5.5 - High temperature: 36°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress : 30°C, pH 5.5, 1M KCl The conditions for Kluyveromyces marxianus CBS6556 are: - Standard condition : 30°C, pH 5.5 - High temperature: 40°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress: 30°C, pH 5.5, 0.6 M KCl The conditions for Yarrowia lipolytica W29 are: - Standard condition: 28°C, pH 5.5 - High temperature: 32°C, pH 5.5 - Low pH: 28°C, pH 3.5 This study is part of the OMICS data generation WP of CHASSY project (European Union’s Horizon 2020 grant agreement No 720824).
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:11:20.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aaron Millan Oropeza
SpeciesList	scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Kluyveromyces marxianus DMKU3-1042; NCBI TaxID: 1003335;
ModificationList	alkylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 03:40:56	ID requested
⏵ 1	2022-02-15 09:11:21	announced

Mill, á, n-Oropeza A, Blein-Nicolas M, Monnet V, Zivy M, Henry C, Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes, 10(1):(2022) [pubmed]

submitter keyword: yeast, LC-MS/MS, shotgun, systems biology, UPS2

Véronique Monnet
contact affiliation	Micalis Institute, INRA, PAPPSO, Université Paris-Saclay, Jouy-en-Josas, France.
contact email	veronique.monnet@inra.fr
lab head
Aaron Millan Oropeza
contact affiliation	INRA
contact email	aaron.millan-oropeza@inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012836
     1. Label: PRIDE project
     2. Name: Approaching absolute quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29