PXD012832

PXD012832 is an original dataset announced via ProteomeXchange.

Title	Analysis of bacterial Type VI Secretion Systems Stoichiometry by Targeted Proteomics
Description	The bacterial Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria, including human pathogens. T6SS is evolutionarily related to the contractile phage tails and assembles as a cell envelope attached organelle. The assembly progresses from formation of a membrane complex to assembly of a baseplate followed by copolymerization of a sheath-tube. Rapid sheath contraction propels the rigid inner tube with associated effectors into target cells. To understand the assembly and stoichiometry of this nanomachine, we applied targeted proteomics to determine the protein abundances of the key T6SS components in three model bacteria. The overall stoichiometry of the components is conserved across species and it agrees with the expected composition of the nanomachine. However, there are also species-specific variations of certain components, which may explain the observed differences in the respective dynamics of T6SS. Furthermore, changes in the protein abundance during different growth conditions point to possible mechanisms of regulation of T6SS dynamics. The combination of live-cell imaging and mass-spectrometry analysis suggests that a baseplate component TssE of V. cholerae might undergo an active proteolysis, which together with an effector protein VasX could be involved in regulation of baseplate dynamics and thus T6SS activity.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:07.648.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012832
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Vibrio sp. 57B112Y11; NCBI TaxID: 1366848;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4); Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 02:11:20	ID requested
1	2019-06-19 01:37:30	announced
2	2019-06-24 03:40:40	announced	Updated publication reference for PubMed record(s): 31197144.
⏵ 3	2023-11-14 08:50:09	announced	2023-11-14: Updated project metadata.

10.6019/PXD012832;

Lin L, Lezan E, Schmidt A, Basler M, Abundance of bacterial Type VI secretion system components measured by targeted proteomics. Nat Commun, 10(1):2584(2019) [pubmed]

submitter keyword: complex stoichiometry, Type VI Secretion Systems,SRM

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012832
     1. Label: PRIDE project
     2. Name: Analysis of bacterial Type VI Secretion Systems Stoichiometry by Targeted Proteomics