PXD012831

PXD012831 is an original dataset announced via ProteomeXchange.

Title	Mus musculus primary CD4+ T cell di-glycine remnant and whole cell proteomics
Description	It is known that ubiquitination is important for T cell receptor (TCR) signaling during T cell activation but the breadth of ubiquitination events triggered during TCR signaling is not completely understood. This dataset utilizes di-glycine remnant profiling combined with mass spectrometry to identify a global landscape of ubiquitination events downstream of the TCR and to quantify changes ubiquitin abundance in response to TCR stimulation. Additionally, whole cell proteomics data were generated to measure protein abundances during TCR stimulation. Mouse primary T cells were isolated, proliferated and either remained resting or stimulated with ???CD3/???CD28 to activate downstream signaling through the TCR and co-stimulatory pathways. Di-glycine remnant profiling and whole cell proteomics was performed on rested cells and cells that had undergone ???CD3/???CD28 TCR stimulation for 4 hours. These data were analyzed to identify the ubiquitination events during TCR activation and to quantify the change in peptide-based ubiquitin abundance and total protein abundance over the course of the 4 hour TCR stimulation. Integration of di-glycine and whole cell proteomics was used to generate protein-specific predictions of whether ubiquitination events downstream of TCR signaling lead to a decrease in associated protein abundance. The analysis of these data suggests that T cell activation leads to an increase in ubiquitination that is not associated with proteasomal or lysosomal degradation.
HostingRepository	PRIDE
AnnounceDate	2019-05-10
AnnouncementXML	Submission_2019-05-10_02:53:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 02:00:27	ID requested
1	2019-05-09 06:27:30	announced
⏵ 2	2019-05-10 02:53:54	announced	Updated project metadata.

Dybas JM, O'Leary CE, Ding H, Spruce LA, Seeholzer SH, Oliver PM, T cells. Nat Immunol, 20(6):747-755(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mus musculus primary CD4+ T cell di-glycine remnant and whole cell proteomics

Steven Seeholzer
contact affiliation	Proteomics Core facility. Children's Hospital of Philadelphia.
contact email	seeholzer@email.chop.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012831

PRIDE project URI

• PRIDE
  1. PXD012831
     1. Label: PRIDE project
     2. Name: Mus musculus primary CD4+ T cell di-glycine remnant and whole cell proteomics