PXD012824

PXD012824 is an original dataset announced via ProteomeXchange.

Title	Proteomics of slow and fast human muscle fibers reveals extensive fiber type-specific adaptations in response to exercise training
Description	Skeletal muscle plays an important role in the health-promoting effects of exercise training, yet the underlying mechanisms are not fully elucidated. Proteomics of skeletal muscle is challenging due to presence of non-muscle tissues and existence of different fiber types confounding the results. This can be circumvented by analysis of pure fibers; however this requires isolation of fibers from fresh tissues. We developed a workflow enabling proteomics analysis of isolated muscle fibers from freeze-dried muscle biopsies and identified >4000 proteins. We investigated effects of exercise training on the pool of slow and fast muscle fibers. Exercise altered expression of >500 proteins irrespective of fiber type covering several metabolic processes, mainly related to mitochondria. Furthermore, exercise training altered proteins involved in regulation of post-translational modifications, transcription, Ca++ signaling, fat, and glucose metabolism in a fiber type-specific manner. Our data serves as a valuable resource for elucidating molecular mechanisms underlying muscle performance and health. Finally, our workflow offers methodological advancement allowing proteomic analyses of already stored freeze-dried human muscle biopsies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:59.513.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	atul shahaji deshmukh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 01:09:51	ID requested
1	2020-12-21 03:41:04	announced
⏵ 2	2024-10-22 05:16:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: transcription, mitochondrial biogenesis, MHC, glycogen, glucose metabolism, proteome,Fiber type

Matthias Mann
contact affiliation	NNF center for protein research, faculty of health and medical sciences. Copenhagen university, copenhagen.
contact email	matthias.mann@cpr.ku.dk
lab head
atul shahaji deshmukh
contact affiliation	Copenhagen University
contact email	atul.deshmukh@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD012824

PRIDE project URI

• PRIDE
  1. PXD012824
     1. Label: PRIDE project
     2. Name: Proteomics of slow and fast human muscle fibers reveals extensive fiber type-specific adaptations in response to exercise training