PXD012823

PXD012823 is an original dataset announced via ProteomeXchange.

Title	Acid stress response of Staphylococcus xylosus elicits changes in the proteome and cellular membrane
Description	Aims: Coagulase negative Staphylococcus xylosus strains are used as starter organisms for sausage fermentation. As those strains have to cope with low pH-values during fermentation, the aim of this study was to identify the acid adaptation mechanisms of S. xylosus TMW 2.1523 previously isolated from salami. Methods and Results: A comparative proteomic study between two different acid tolerant mutants was performed. Therefore, both S. xylosus mutants were grown pH-static under acid stress (pH 5.1) and reference conditions (pH 7.0). Proteomic data were supported by metabolite and cell membrane lipids analyses. S. xylosus acid stress adaptation is mainly characterized by a metabolic change towards neutral metabolites, enhanced urease activity, reduced ATP consumption, an increase in membrane fluidity and changes of the membrane thickness. Conclusion: This study corroborates mechanisms as previously described for other Gram-positive bacteria. Additionally, the adjustment of membrane structure and composition in S. xylosus TMW 2.1523 play a prominent role in its acid adaptation. Significance and Impact of the Study: This study demonstrates for the first time changes of the membrane lipid composition due to acid stress adaptation in staphylococci.
HostingRepository	PRIDE
AnnounceDate	2019-02-23
AnnouncementXML	Submission_2019-05-27_01:07:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Staphylococcus xylosus; NCBI TaxID: 1288;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-22 01:07:03	ID requested
1	2019-02-22 01:50:52	announced
2	2019-02-23 00:30:37	announced	Updated project metadata.
⏵ 3	2019-05-27 01:07:53	announced	Updated project metadata.

Kolbeck S, Behr J, Vogel RF, Ludwig C, Ehrmann MA, Acid stress response of Staphylococcus xylosus elicits changes in the proteome and cellular membrane. J Appl Microbiol, 126(5):1480-1495(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Sausage fermentation, acid adaptation, staphylococci, proteomic, fatty acids, UV-mutation

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry Technische Universität München Gregor-Mendel-Straße 4 85354 Freising GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012823
     1. Label: PRIDE project
     2. Name: Acid stress response of Staphylococcus xylosus elicits changes in the proteome and cellular membrane