PXD012815

PXD012815 is an original dataset announced via ProteomeXchange.

Title	An essential role for cullin 3 ubiquitin ligases and phototropin 2 turnover in seedling chloroplast development (de-etiolation)
Description	Transgenic Arabidopsis seedlings RV86-5 (contains a dexamethasone-inducible ubiquitin variant that contains Arg instead of Lys at position 48; ̈Schlögelhofer et al., 2005) were surface-sterilized and sown on Uhelon 120T (Silk & Progress, Czech Republic) mesh placed on 1% (w/v) agar containing a half-strength Murashige and Skoog medium (pH 5.7), stratified at 4 °C for 3 d, and cultivated at 21 °C/19 °C day/night temperatures, with a 16 h photoperiod (90 μmol m−2 s−1 light intensity) for 14 d. On the fourteenth day (after the first 2 h of the day period), the Uhelon mesh with the seedlings was transferred onto a new solid medium supplemented with (i) 5×10−4% (v/v) DMSO (mock); (ii) 0.7 or 7.0 μM dexamethasone (DEX) and rinsed with DMSO or DEX-supplemented water for mock and DEX-treated seedlings, respectively, and incubated for 24 hours.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:07.010.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-21 02:01:35	ID requested
1	2022-05-04 04:13:20	announced
⏵ 2	2023-11-14 08:50:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Arabidopsis, development, protein turnover, phototropin

Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD012815

PRIDE project URI

• PRIDE
  1. PXD012815
     1. Label: PRIDE project
     2. Name: An essential role for cullin 3 ubiquitin ligases and phototropin 2 turnover in seedling chloroplast development (de-etiolation)