PXD012799

PXD012799 is an original dataset announced via ProteomeXchange.

Title	An Archaeal Pilus Survives the Most Extreme Conditions Through Extensive Glycosylation
Description	Pili on the surface of Sulfolobus islandicus are used for a host of functions, and serve as receptors for certain archaeal viruses. We find that these pili, when removed from cells, resist digestion by trypsin or pepsin, and survive boiling in SDS or 5M guanidinium-HCl. We have used cryo-EM to determine the structure of these filaments at 4.1 Å resolution. An atomic model was built by combining the map with bioinformatics without prior knowledge of the pilin sequence, an approach that should prove useful when looking at assemblies where all of the components may not be known. The atomic structure of the archaeal pilus was unusual due to almost a third of the residues being either threonine or serine and many hydrophobic surface residues. While the map showed specific glycosylation of only three residues, mass per unit length measurements suggested extensive glycosylation. We show that this extensive glycosylation renders these filaments soluble and provides the remarkable structural stability. We also show that the overall fold of the archaeal pilin is quite similar to archaeal flagellin, establishing common evolutionary origins.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_13:24:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JJ Park
SpeciesList	scientific name: Sulfolobus islandicus LAL14/1; NCBI TaxID: 1241935;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-20 06:45:29	ID requested
⏵ 1	2020-05-26 13:24:23	announced

Wang F, Cvirkaite-Krupovic V, Kreutzberger MAB, Su Z, de Oliveira GAP, Osinski T, Sherman N, DiMaio F, Wall JS, Prangishvili D, Krupovic M, Egelman EH, An extensively glycosylated archaeal pilus survives extreme conditions. Nat Microbiol, 4(8):1401-1410(2019) [pubmed]

submitter keyword: Pili, Cryo-EM, glycosylation, LC-MS

Edward H. Egelman
contact affiliation	Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA
contact email	egelman@virginia.edu
lab head
JJ Park
contact affiliation	University of Virginia
contact email	jp2ht@virginia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD012799

PRIDE project URI

• PRIDE
  1. PXD012799
     1. Label: PRIDE project
     2. Name: An Archaeal Pilus Survives the Most Extreme Conditions Through Extensive Glycosylation