PXD012795

PXD012795 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Raptor-proximal proteins in U2OS cells
Description	The mammalian target of rapamycin complex 1 (mTORC1) is a key nutrient-sensing hub and a master regulator of cellular growth and metabolism. Activation of mTORC1 by amino acids and growth factors requires its recruitment to the lysosomes. Furthermore, lysosomal distribution has been shown to be intimately involved in the control of mTORC1, where localisation of lysosomes near plasma membrane increases mTORC1 activity. However, the underlying mechanisms by which lysosomal positioning impacts on mTORC1 signalling are not well understood. To understand better the spatial associations of mTORC1, we analysed the proximal ‘interactome’ of the constitutive component of mTORC1, Raptor, using BioID2-based proximity labelling and MS-based proteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:25:53.197.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Byron
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-20 03:51:16	ID requested
1	2021-09-08 09:09:16	announced
⏵ 2	2024-10-22 05:25:55	announced	2024-10-22: Updated project metadata.

Rabanal-Ruiz Y, Byron A, Wirth A, Madsen R, Sedlackova L, Hewitt G, Nelson G, Stingele J, Wills JC, Zhang T, Zeug A, F, ä, ssler R, Vanhaesebroeck B, Maddocks ODK, Ponimaskin E, Carroll B, Korolchuk VI, mTORC1 activity is supported by spatial association with focal adhesions. J Cell Biol, 220(5):(2021) [pubmed]

10.1083/jcb.202004010;

submitter keyword: mTORC1, Raptor, MS, Q Exactive Plus, proximity labelling, proximity-based biotinylation,human, BioID, BioID2, osteosarcoma, LC-MS/MS

Viktor I Korolchuk
contact affiliation	Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom
contact email	viktor.korolchuk@newcastle.ac.uk
lab head
Adam Byron
contact affiliation	University of Manchester
contact email	adam.byron@igmm.ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD012795

PRIDE project URI

• PRIDE
  1. PXD012795
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Raptor-proximal proteins in U2OS cells