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PXD012785

PXD012785 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative temporal proteomic analysis of vaccinia virus infection reveals regulation of histone deacetylases by an interferon antagonist
DescriptionVaccinia virus (VACV) has numerous immune evasion strategies, including multiple mechanisms of inhibition of IRF-3, NF-κB and type I interferon (IFN) signaling. Here, we used highly multiplexed proteomics to quantify >8,000 cellular proteins and ~80% of viral proteins over seven time points spanning the whole course of VACV infection. This identified multiple novel viral targets, including putative natural killer cell ligands and IFN-stimulated genes. The class II histone deacetylase HDAC5 was selectively degraded early during VACV infection. Use of cell lines in which HDAC5 was overexpressed or knocked out showed that HDAC5 restricted replication of both VACV and herpes simplex virus type 1 (HSV-1). By generating a protein-based temporal classification of VACV gene expression, we identified the early protein C6, a multifunctional IFN antagonist, as the factor that targets HDAC5 for proteasomal degradation. Our approach thus identifies both a novel restriction factor and a viral mechanism of innate immune evasion.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:53:57.297.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Weekes
SpeciesList scientific name: Vaccinia virus Western Reserve; NCBI TaxID: 696871; scientific name: Vaccinia virus; NCBI TaxID: NCBITaxon:10245; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-19 05:22:32ID requested
12019-05-07 09:03:48announced
22019-05-27 00:08:49announcedUpdated publication reference for PubMed record(s): 31067474.
32024-10-22 04:53:58announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2019.04.042;
Soday L, Lu Y, Albarnaz JD, Davies CTR, Antrobus R, Smith GL, Weekes MP, Quantitative Temporal Proteomic Analysis of Vaccinia Virus Infection Reveals Regulation of Histone Deacetylases by an Interferon Antagonist. Cell Rep, 27(6):1920-1933.e7(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: quantitative proteomics
tandem mass tag
systems virology
poxvirus
interferon
host-pathogen interaction
immune evasion
innate immunity
restriction factor
histone deacetylase
Contact List
Michael Weekes
contact affiliationCambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
contact emailMpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliationUniversity of Cambridge
contact emailmpw1001@cam.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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