PXD012749

PXD012749 is an original dataset announced via ProteomeXchange.

Title	Amorphous TiO2 Nanotubes as Platform for Highly Selective Phos-phopeptide Enrichment
Description	This work reports highly selective phosphopeptide enrichment using amorphous TiO2 nanotubes (TiO2NTs) and the same material decorated with superparamagnetic Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs). TiO2NTs and TiO2NTs@Fe3O4NPs materials were applied for phosphopeptide enrichment both from a simple peptide mixture (tryptic digest of bovine serum albumin and α-casein) as well as from a complex peptide mixture (tryptic digest of Jurkat T cell lysate). The obtained enrichment efficiency and selectivi-ty for phosphopeptides of TiO2NTs and TiO2NTs@Fe3O4NPs was excellent compared to those of the well-established TiO2 micro-spheres. The enrichment protocol was extended for a second elution step facilitating the identification of additional phosphopep-tides. It further turned out that both types of amorphous TiO2 nanotubes provide qualitatively new physico-chemical features that are clearly advantageous for highly selective phosphopeptide enrichment. This has been confirmed experimentally resulting in substantial reduction of non-phosphorylated peptides in the enriched samples. In addition, TiO2NTs@Fe3O4NPs combine a high selectivity with ease of handling due to the superparamagnetic character of the material. The presented materials and performances are further promising for applications towards a whole range of other types of biomolecules to be treated in similar fashion.
HostingRepository	PRIDE
AnnounceDate	2019-10-11
AnnouncementXML	Submission_2019-10-11_07:54:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012749
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pavel Rehulka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Dehydrated; Oxidation; Acetyl; Acetyl; Carbamidomethyl; Gln->pyro-Glu; Pyro-carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-18 01:50:40	ID requested
1	2019-06-26 00:43:30	announced
⏵ 2	2019-10-11 07:54:51	announced	2019-10-11: Updated project metadata.

Kupcik R, Macak JM, Rehulkova H, Sopha H, Fabrik I, Anitha VC, Klimentova J, Murasova P, Bilkova Z, Rehulka P, Nanotubes as a Platform for Highly Selective Phosphopeptide Enrichment. ACS Omega, 4(7):12156-12166(2019) [pubmed]

curator keyword: Technical

submitter keyword: phosphopeptide enrichment, nanotubes

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology Faculty of Military Health Sciences, University of Defence Trebesska 1575, 50001 Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Pavel Rehulka
contact affiliation	Department of Molecular Pathology and Biology FMHS UD, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic
contact email	pavel.rehulka@unob.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012749

PRIDE project URI

• PRIDE
  1. PXD012749
     1. Label: PRIDE project
     2. Name: Amorphous TiO2 Nanotubes as Platform for Highly Selective Phos-phopeptide Enrichment