PXD012743 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis |
Description | Mitochondrial proteins are replete with phosphorylation; however, the origin, abundance, and functional relevance of these modifications are largely unclear. Nonetheless, mitochondria possess multiple resident phosphatases, suggesting that protein dephosphorylation may be broadly important for mitochondrial activities. To explore this, we deleted the poorly characterized matrix phosphatase Pptc7 from mice using CRISPR-Cas9 technology. Strikingly, Pptc7-/- mice exhibited marked hypoketotic hypoglycemia, elevated acylcarnitines, and lactic acidosis, and died soon after birth. Pptc7-/- tissues had significantly diminished mitochondrial size and protein content despite normal transcript levels, but consistently elevated phosphorylation on select mitochondrial proteins. These putative Pptc7 substrates include the protein translocase complex subunit Timm50, whose phosphorylation reduced import activity. We further find that phosphorylation in or near the mitochondrial targeting sequences of multiple proteins can disrupt their import rates and matrix processing. Overall, our data define Pptc7 as a protein phosphatase essential for proper mitochondrial function and biogenesis during the extrauterine transition. Natalie M Niemi, Gary M Wilson, Katherine A Overmyer, F.-Nora Vogtle, Danielle C Lohman, Kathryn L Schueler, Alan D Attie, Chris Meisinger, Joshua J Coon, David J Pagliarini. (2018) “Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis.” biorx: https://doi.org/10.1101/426247 |
HostingRepository | PRIDE |
AnnounceDate | 2019-05-29 |
AnnouncementXML | Submission_2019-07-29_01:06:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gary Wilson |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-18 01:21:53 | ID requested | |
1 | 2019-05-29 00:56:41 | announced | |
⏵ 2 | 2019-07-29 01:06:25 | announced | Updated publication reference for PubMed record(s): 31324765. |
Publication List
Niemi NM, Wilson GM, Overmyer KA, V, ö, gtle FN, Myketin L, Lohman DC, Schueler KL, Attie AD, Meisinger C, Coon JJ, Pagliarini DJ, Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis. Nat Commun, 10(1):3197(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Pptc7, Mitochondia, Phosphoproteome |
Contact List
Joshua J. Coon |
contact affiliation | University of Wisconsin-Madison |
contact email | jcoon@chem.wisc.edu |
lab head | |
Gary Wilson |
contact affiliation | UW-Madison |
contact email | gwilson9@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012743
- Label: PRIDE project
- Name: Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis