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PXD012743

PXD012743 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis
DescriptionMitochondrial proteins are replete with phosphorylation; however, the origin, abundance, and functional relevance of these modifications are largely unclear. Nonetheless, mitochondria possess multiple resident phosphatases, suggesting that protein dephosphorylation may be broadly important for mitochondrial activities. To explore this, we deleted the poorly characterized matrix phosphatase Pptc7 from mice using CRISPR-Cas9 technology. Strikingly, Pptc7-/- mice exhibited marked hypoketotic hypoglycemia, elevated acylcarnitines, and lactic acidosis, and died soon after birth. Pptc7-/- tissues had significantly diminished mitochondrial size and protein content despite normal transcript levels, but consistently elevated phosphorylation on select mitochondrial proteins. These putative Pptc7 substrates include the protein translocase complex subunit Timm50, whose phosphorylation reduced import activity. We further find that phosphorylation in or near the mitochondrial targeting sequences of multiple proteins can disrupt their import rates and matrix processing. Overall, our data define Pptc7 as a protein phosphatase essential for proper mitochondrial function and biogenesis during the extrauterine transition. Natalie M Niemi, Gary M Wilson, Katherine A Overmyer, F.-Nora Vogtle, Danielle C Lohman, Kathryn L Schueler, Alan D Attie, Chris Meisinger, Joshua J Coon, David J Pagliarini. (2018) “Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis.” biorx: https://doi.org/10.1101/426247
HostingRepositoryPRIDE
AnnounceDate2019-05-29
AnnouncementXMLSubmission_2019-07-29_01:06:24.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGary Wilson
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-18 01:21:53ID requested
12019-05-29 00:56:41announced
22019-07-29 01:06:25announcedUpdated publication reference for PubMed record(s): 31324765.
Publication List
Niemi NM, Wilson GM, Overmyer KA, V, ö, gtle FN, Myketin L, Lohman DC, Schueler KL, Attie AD, Meisinger C, Coon JJ, Pagliarini DJ, Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis. Nat Commun, 10(1):3197(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Pptc7, Mitochondia, Phosphoproteome
Contact List
Joshua J. Coon
contact affiliationUniversity of Wisconsin-Madison
contact emailjcoon@chem.wisc.edu
lab head
Gary Wilson
contact affiliationUW-Madison
contact emailgwilson9@wisc.edu
dataset submitter
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Dataset FTP location
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