PXD012734

PXD012734 is an original dataset announced via ProteomeXchange.

Title	Identification of mitotic substrates of phospho-regulated USP9X
Description	We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732) and subsequently identified USP9X as a specific CDC14B target (PXD012733). To investigate relevant mitotic substrates of phospho-regulated USP9X, we next performed a Stable isotope labeling by amino acids in cell culture (SILAC)-based screen in which ubiquitylated proteins were purified from control or USP9X depleted HEK 293T cells that were either asynchronous or synchronized in mitosis. Stable expression of recombinant His-tagged ubiquitin has been used for selective enrichment of ubiquitinylated proteins in both conditions.
HostingRepository	PRIDE
AnnounceDate	2020-01-24
AnnouncementXML	Submission_2020-03-17_01:54:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Gloeckner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-15 06:06:12	ID requested
1	2020-01-24 00:20:59	announced
⏵ 2	2020-03-17 01:54:41	announced	2020-03-17: Updated publication reference for PubMed record(s): 32152317.

Dietachmayr M, Rathakrishnan A, Karpiuk O, von Zweydorf F, Engleitner T, Fern, á, ndez-S, á, iz V, Schenk P, Ueffing M, Rad R, Eilers M, Gloeckner CJ, Clemm von Hohenberg K, Bassermann F, Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival. Nat Commun, 11(1):1268(2020) [pubmed]

curator keyword: Biological

submitter keyword: USP9X, ubiquitinylation

Christian Johannes Gloeckner
contact affiliation	(1) German Center for Neurodegenerative Diseases (DZNE), 72076 Tübingen, Germany (2) University of Tübingen, Center for Ophthalmology, Institute for Ophthalmic Research, 72074 Tübingen, Germany
contact email	johannes.gloeckner@dzne.de
lab head
Johannes Gloeckner
contact affiliation	DZNE
contact email	johannes.gloeckner@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD012734

PRIDE project URI

• PRIDE
  1. PXD012734
     1. Label: PRIDE project
     2. Name: Identification of mitotic substrates of phospho-regulated USP9X