PXD012717

PXD012717 is an original dataset announced via ProteomeXchange.

Title	A heterochromatin specific RNA export pathway facilitates piRNA production
Description	PIWI-interacting RNAs (piRNAs), a class of small RNAs that guide transposon silencing in animals, are processed in the cytoplasm from RNA Polymerase II transcripts. How piRNA precursors, which often lack RNA maturation signatures and thereby violate quality control checkpoints, achieve nuclear export is unknown. Here, we uncover a germline-specific RNA export pathway in Drosophila, that escorts piRNA precursors from their heterochromatic origins to nuage, the cytoplasmic piRNA processing centres. This pathway connects canonical nuclear export factors—the RNA helicase UAP56, the NXF cofactor Nxt1/p15, and the exportin Crm1/Xpo1—with Nxf3, a variant of the mRNA exporter Nxf1/Tap. Nxf3 recruitment to nascent piRNA precursors occurs via the heterochromatin protein 1-variant Rhino and CG13741/Bootlegger, a new piRNA pathway factor, thereby making piRNA precursor export independent of RNA processing events. Thus, similar to retroviral hijacking of cellular export factors, piRNA precursor export evolved to bend canonical gene expression rules through bypassing nuclear RNA surveillance mechanisms.
HostingRepository	PRIDE
AnnounceDate	2019-03-29
AnnouncementXML	Submission_2019-08-16_00:24:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-14 07:31:37	ID requested
1	2019-03-29 07:37:11	announced
⏵ 2	2019-08-16 00:24:42	announced	Updated publication reference for PubMed record(s): 31398345.

ElMaghraby MF, Andersen PR, P, ü, hringer F, Hohmann U, Meixner K, Lendl T, Tirian L, Brennecke J, A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production. Cell, 178(4):964-979.e20(2019) [pubmed]

curator keyword: Biological

submitter keyword: Drosophila, Ovary, piRNA, export

Julius Fabian Brennecke
contact affiliation	IMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
contact email	julius.brennecke@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA - Institute of Molecular Biotechnology
contact email	imre@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012717

PRIDE project URI

• PRIDE
  1. PXD012717
     1. Label: PRIDE project
     2. Name: A heterochromatin specific RNA export pathway facilitates piRNA production