PXD012716

PXD012716 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	streptococcus suis LC-MS/MS - MnmE, a Central tRNA-Modifying GTPase, Is Essential for the Growth, Pathogenicity, and Arginine Metabolism of Streptococcus suis Serotype 2
Description	Streptococcus suis is an important pathogen in pigs and can also cause severe infections in humans. However, little is known about proteins associated with cell growth and virulence of S. suis. In this study, a GTPase MnmE homologue was identified in a Chinese isolate (SC19) that drives a tRNA modification reaction. A mnmE deletion strain (ΔmnmE) and a complementary strain (CΔmnmE) were constructed to systematically decode the characteristics and the function of MnmE by applying in vitro and in vivo studies, and proteomic analysis. Phenotypic analysis revealed that the ΔmnmE strain displayed deficient growth, attenuated pathogenicity, perturbed arginine metabolic pathway mediated by arginine deiminase system (ADS). Consistently, TMT-based quantitative proteomics analysis confirmed that 491 proteins were differentially expressed (238 up- and 253 down-regulated) between strains ΔmnmE and SC19. Many proteins associated with DNA replication, cell division, and virulence were down-regulated. Particularly, the core enzymes of ADS were significantly down-regulated in strain ΔmnmE. These data also provided putative molecular mechanisms for MnmE in cell growth and survival in an acidic environment. Therefore, MnmE is a central regulator that plays an important role in cell growth and virulence, as well as arginine metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:52.168.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ting gao
SpeciesList	scientific name: Streptococcus suis SC149; NCBI TaxID: 1323674;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-14 06:09:30	ID requested
1	2021-03-04 14:04:02	announced
⏵ 2	2024-10-22 04:52:53	announced	2024-10-22: Updated project metadata.

Publication List

Gao T, Yuan F, Liu Z, Liu W, Zhou D, Yang K, Duan Z, Guo R, Liang W, Hu Q, Tian Y, Zhou R, Serotype 2. Front Cell Infect Microbiol, 9():173(2019) [pubmed]
10.3389/fcimb.2019.00173;

Gao T, Yuan F, Liu Z, Liu W, Zhou D, Yang K, Duan Z, Guo R, Liang W, Hu Q, Tian Y, Zhou R, Serotype 2. Front Cell Infect Microbiol, 9():173(2019) [pubmed]

10.3389/fcimb.2019.00173;

Keyword List

submitter keyword: SS2,TMT,MnmE

submitter keyword: SS2,TMT,MnmE

Contact List

gao ting
contact affiliation	hubei academy of agricultural sciences
contact email	gaotingyefeiyeziyu@163.com
lab head
ting gao
contact affiliation	company
contact email	gaotingyefeiyeziyu@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012716
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012716

PRIDE project URI

Repository Record List

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