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PXD012716

PXD012716 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlestreptococcus suis LC-MS/MS - MnmE, a Central tRNA-Modifying GTPase, Is Essential for the Growth, Pathogenicity, and Arginine Metabolism of Streptococcus suis Serotype 2
DescriptionStreptococcus suis is an important pathogen in pigs and can also cause severe infections in humans. However, little is known about proteins associated with cell growth and virulence of S. suis. In this study, a GTPase MnmE homologue was identified in a Chinese isolate (SC19) that drives a tRNA modification reaction. A mnmE deletion strain (ΔmnmE) and a complementary strain (CΔmnmE) were constructed to systematically decode the characteristics and the function of MnmE by applying in vitro and in vivo studies, and proteomic analysis. Phenotypic analysis revealed that the ΔmnmE strain displayed deficient growth, attenuated pathogenicity, perturbed arginine metabolic pathway mediated by arginine deiminase system (ADS). Consistently, TMT-based quantitative proteomics analysis confirmed that 491 proteins were differentially expressed (238 up- and 253 down-regulated) between strains ΔmnmE and SC19. Many proteins associated with DNA replication, cell division, and virulence were down-regulated. Particularly, the core enzymes of ADS were significantly down-regulated in strain ΔmnmE. These data also provided putative molecular mechanisms for MnmE in cell growth and survival in an acidic environment. Therefore, MnmE is a central regulator that plays an important role in cell growth and virulence, as well as arginine metabolism.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:52:52.168.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterting gao
SpeciesList scientific name: Streptococcus suis SC149; NCBI TaxID: 1323674;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-14 06:09:30ID requested
12021-03-04 14:04:02announced
22024-10-22 04:52:53announced2024-10-22: Updated project metadata.
Publication List
Gao T, Yuan F, Liu Z, Liu W, Zhou D, Yang K, Duan Z, Guo R, Liang W, Hu Q, Tian Y, Zhou R, Serotype 2. Front Cell Infect Microbiol, 9():173(2019) [pubmed]
10.3389/fcimb.2019.00173;
Keyword List
submitter keyword: SS2,TMT,MnmE
Contact List
gao ting
contact affiliationhubei academy of agricultural sciences
contact emailgaotingyefeiyeziyu@163.com
lab head
ting gao
contact affiliationcompany
contact emailgaotingyefeiyeziyu@163.com
dataset submitter
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Dataset FTP location
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