PXD012715
PXD012715 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | BRD4 IP MS in leukemia - MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation |
Description | The histone acetyl-reader BRD4 is an important regulator of chromatin structure and transcription, yet factors modulating its activity have remained elusive. Here we describe two complementary screens for genetic and physical interactors of BRD4, which converge on the folate pathway enzyme MTHFD1. We show that a fraction of MTHFD1 resides in the nucleus, where it is recruited to distinct genomic loci by direct interaction with BRD4. Inhibition of either BRD4 or MTHFD1 results in similar changes in nuclear metabolite composition and gene expression, and pharmacologic inhibitors of the two pathways synergize to impair cancer cell viability in vitro and in vivo. Our finding that MTHFD1 and other metabolic enzymes are chromatin-associated suggests a direct role for nuclear metabolism in the control of gene expression. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:52:52.165.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Richard Imre |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-02-14 05:35:57 | ID requested | |
1 | 2019-04-04 00:01:43 | announced | |
2 | 2019-12-13 04:00:11 | announced | 2019-12-13: Updated publication reference for PubMed record(s): 31133746. |
⏵ 3 | 2024-10-22 04:52:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41588-019-0413-z; |
Sdelci S, Rendeiro AF, Rathert P, You W, Lin JG, Ringler A, Hofst, ä, tter G, Moll HP, G, ü, rtl B, Farlik M, Schick S, Klepsch F, Oldach M, Buphamalai P, Schischlik F, M, á, jek P, Parapatics K, Schmidl C, Schuster M, Penz T, Buckley DL, Hudecz O, Imre R, Wang SY, Maric HM, Kralovics R, Bennett KL, M, ü, ller AC, Mechtler K, Menche J, Bradner JE, Winter GE, Klavins K, Casanova E, Bock C, Zuber J, Kubicek S, MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet, 51(6):990-998(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: leukemia, MTHFD1, complex,BRD4 |
Contact List
Karl Mechtler | |
---|---|
contact affiliation | Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 1030 Vienna, Austria |
contact email | karl.mechtler@imp.ac.at |
lab head | |
Richard Imre | |
contact affiliation | IMBA - Institute of Molecular Biotechnology |
contact email | imre@imp.ac.at |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012715 |
PRIDE project URI |
Repository Record List
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