PXD012712

PXD012712 is an original dataset announced via ProteomeXchange.

Title	Absolute quantification of cohesin and its regulators in human cells
Description	The organisation of mammalian genomes into loops and topologically associated domains (TADs) regulates gene expression. The formation and maintenance of loops and TADs depends on the protein complex cohesin, which also holds replicated sister chromatids together from S phase until mitosis. To understand how cohesin functions, it is essential to know how many cohesin complexes and regulators exist in a typical cell and how they are distributed genome-wide. Here, we have used quantitative mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching to measure the absolute number and dynamics of core subunits and regulators of the cohesin complex in human cells. We find that 60000 – 134000 cohesin complexes are bound to chromatin per G1 HeLa cell, suggesting that cohesin most likely does not occupy all its binding sites concurrently. We incorporate our data into mathematical models and discuss implications for how cohesin might contribute to genome organisation and sister chromatid cohesion.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:22.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Dürnberger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q TRAP; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-14 03:41:24	ID requested
1	2019-11-12 18:45:30	announced
⏵ 2	2024-10-22 04:53:30	announced	2024-10-22: Updated project metadata.

10.7554/eLife.46269;

Holzmann J, Politi AZ, Nagasaka K, Hantsche-Grininger M, Walther N, Koch B, Fuchs J, D, ü, rnberger G, Tang W, Ladurner R, Stocsits RR, Busslinger GA, Nov, á, k B, Mechtler K, Davidson IF, Ellenberg J, Peters JM, Absolute quantification of cohesin, CTCF and their regulators in human cells. Elife, 8():(2019) [pubmed]

submitter keyword: fluorescence-correlation spectroscopy, mass spectrometry,Cell cycle, genome organization, cohesin, sister chromatid cohesion

Jan-Michael Peters
contact affiliation	IMP - Research Institute of Molecular Pathology, Vienna, Austria
contact email	jan-michael.peters@imp.ac.at
lab head
Gerhard Dürnberger
contact affiliation	GMI/IMP/IMBA
contact email	gerhard.duernberger@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012712

PRIDE project URI

• PRIDE
  1. PXD012712
     1. Label: PRIDE project
     2. Name: Absolute quantification of cohesin and its regulators in human cells