PXD012701 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phytoplasma induced changes in acetylatome and succinylatome provide evidence for involvement of acetylated proteins in Paulownia witches’ broom |
Description | Lysine acetylation and succinylation are post-translational modifications of proteins, and have been shown to play roles in plant response to pathogen infection. Phytoplasma infection can directly alter multiple metabolic processes in Paulownia and lead to Paulownia witches’ broom (PaWB), the major cause of Paulownia mortality worldwide. To explore the extent and function of lysine acylations during phytoplasma infection, we investigated global proteome, acetylome, and succinylome of phytoplasma-infected Paulownia tomentosa seedlings. In total, we globally yield 8963 proteins, 2893 acetylated, and 1271 succinylated proteins. Among them, 425 substrates were simultaneously acetylated and succinylated. Comparative analysis revealed that 276 proteins, 546 acetylated proteins and 5 succinylated proteins were associated with PaWB. Our results suggested that acetylation may be more important than succinylation in response to phytoplasma infection. Enzymatic assays showed that acetylation modified the activities of protochlorophyllide reductase and RuBisCO in phytoplasma-infected seedlings. On the basis of these results, a model to elucidate the molecular mechanism responses to PaWB was proposed and this research offer a resource for functional studies on the effects of acetylation on protein function. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:53:13.493.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | xiaomei li |
SpeciesList | scientific name: Paulownia; NCBI TaxID: 39251; |
ModificationList | succinylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-14 02:00:43 | ID requested | |
1 | 2019-04-04 00:42:44 | announced | |
⏵ 2 | 2024-10-22 04:53:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra118.001104; |
Cao Y, Fan G, Wang Z, Gu Z, Provide Evidence for Involvement of Acetylated Proteins in Witches' Broom Disease. Mol Cell Proteomics, 18(6):1210-1226(2019) [pubmed] |
Keyword List
submitter keyword: succinylation,LC-MS/MS, Paulownia,Acetylation |
Contact List
Guoqiang Fan |
contact affiliation | Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan, 450002, P. R. China |
contact email | zlxx64@126.com |
lab head | |
xiaomei li |
contact affiliation | PTM Biolabs lnc. |
contact email | xiaomei_li@ptm-biolab.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012701 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012701
- Label: PRIDE project
- Name: Phytoplasma induced changes in acetylatome and succinylatome provide evidence for involvement of acetylated proteins in Paulownia witches’ broom