PXD012701

PXD012701 is an original dataset announced via ProteomeXchange.

Title	Phytoplasma induced changes in acetylatome and succinylatome provide evidence for involvement of acetylated proteins in Paulownia witches’ broom
Description	Lysine acetylation and succinylation are post-translational modifications of proteins, and have been shown to play roles in plant response to pathogen infection. Phytoplasma infection can directly alter multiple metabolic processes in Paulownia and lead to Paulownia witches’ broom (PaWB), the major cause of Paulownia mortality worldwide. To explore the extent and function of lysine acylations during phytoplasma infection, we investigated global proteome, acetylome, and succinylome of phytoplasma-infected Paulownia tomentosa seedlings. In total, we globally yield 8963 proteins, 2893 acetylated, and 1271 succinylated proteins. Among them, 425 substrates were simultaneously acetylated and succinylated. Comparative analysis revealed that 276 proteins, 546 acetylated proteins and 5 succinylated proteins were associated with PaWB. Our results suggested that acetylation may be more important than succinylation in response to phytoplasma infection. Enzymatic assays showed that acetylation modified the activities of protochlorophyllide reductase and RuBisCO in phytoplasma-infected seedlings. On the basis of these results, a model to elucidate the molecular mechanism responses to PaWB was proposed and this research offer a resource for functional studies on the effects of acetylation on protein function.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:13.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaomei li
SpeciesList	scientific name: Paulownia; NCBI TaxID: 39251;
ModificationList	succinylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-14 02:00:43	ID requested
1	2019-04-04 00:42:44	announced
⏵ 2	2024-10-22 04:53:15	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra118.001104;

Cao Y, Fan G, Wang Z, Gu Z, Provide Evidence for Involvement of Acetylated Proteins in Witches' Broom Disease. Mol Cell Proteomics, 18(6):1210-1226(2019) [pubmed]

submitter keyword: succinylation,LC-MS/MS, Paulownia,Acetylation

Guoqiang Fan
contact affiliation	Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan, 450002, P. R. China
contact email	zlxx64@126.com
lab head
xiaomei li
contact affiliation	PTM Biolabs lnc.
contact email	xiaomei_li@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012701
     1. Label: PRIDE project
     2. Name: Phytoplasma induced changes in acetylatome and succinylatome provide evidence for involvement of acetylated proteins in Paulownia witches’ broom