PXD012698

PXD012698 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomic analysis of DNA methyltransferase mutants of the cyanobacterium Synechocystis sp. PCC 6803 – Analysis of the membrane-enriched fraction
Description	Total protein and membrane-enriched fractions of Synechocystis sp. PCC 6803 wild type (WT) and mutant strains affected with inactivated DNA methyltransferase genes were analyzed by LC-HDMSE. The mutation of the DNA methyltransferase gene sll0729 encoding M.Ssp6803II initially led to a strong phenotype, including a lowered chlorophyll/phycocyanin ratio, impaired growth, and alterations in gene expression. Prolonged cultivation revealed instability of the initially obtained phenotype. Colonies showing normal pigmentation and WT-like growth appeared regularly and in high frequencies on agar plates. These colonies represent suppressor mutants, since the sll0729 gene is still completely inactivated and methylation of the M.Ssp6803II target GGCC sites does not occur. The proteomic analysis comprises the WT, two strains of suppressor mutants sll0729_1 and sll0729_15 as well as mutants of the DNA methyltransferase genes slr6095 and sll8009. For each of these five strains we investigated three biological replicates. Comparisons between the WT and these two suppressor mutant strains, but also between them and the slr6095 and sll8009 mutants enabled the detection of expression differences, which are specifically linked to the absence of M.Ssp6803II-related DNA methylation.
HostingRepository	PRIDE
AnnounceDate	2019-06-26
AnnouncementXML	Submission_2019-06-26_00:30:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012698
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stefan Mikkat
SpeciesList	scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-13 23:48:26	ID requested
⏵ 1	2019-06-26 00:30:32	announced

G, ä, rtner K, Kl, ä, hn S, Watanabe S, Mikkat S, Scholz I, Hess WR, Hagemann M, sp. PCC 6803. Front Microbiol, 10():1233(2019) [pubmed]

curator keyword: Biological

submitter keyword: Synechocystis sp. PCC 6803, DNA methyltransferase, suppressor mutants, HDMSE, label-free quantification

Martin Hagemann
contact affiliation	Department of Plant Physiology, University of Rostock, Germany
contact email	martin.hagemann@uni-rostock.de
lab head
Stefan Mikkat
contact affiliation	Core Facility Proteome Analysis, University Medicine Rostock, Schillingallee 69, 18057 Rostock
contact email	stefan.mikkat@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012698
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomic analysis of DNA methyltransferase mutants of the cyanobacterium Synechocystis sp. PCC 6803 – Analysis of the membrane-enriched fraction