PXD012693

PXD012693 is an original dataset announced via ProteomeXchange.

Title	Pax3 interactome in mouse ES cell-derived mesodermal precursors
Description	The skeletal myogenic lineage in developing embryos is specified within the somites and requires members of the Paired box (Pax) family of transcription factors. In particular, Pax3 is the first member of this family expressed in the central region of the dermomyotome and it is necessary for specification, migration and survival of the myogenic progenitors that will form the muscles of the limbs, diaphragm and tongue. To investigate Pax3 molecular functions in the developing mesoderm, we generated doxycycline-inducible mouse embryonic stem (ES) cells expressing untagged and HaFlag-tagged-Pax3. These cell lines were used to unbiasedly identify its interacting partners by tandem affinity purification followed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-04-18
AnnouncementXML	Submission_2019-05-31_06:08:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Magli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-13 06:41:14	ID requested
1	2019-04-18 07:47:21	announced
⏵ 2	2019-05-31 06:08:49	announced	Updated publication reference for PubMed record(s): 31127120.

Magli A, Baik J, Pota P, Cordero CO, Kwak IY, Garry DJ, Love PE, Dynlacht BD, Perlingeiro RCR, Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification. Nat Commun, 10(1):2316(2019) [pubmed]

submitter keyword: Pax3, mesoderm, LC-MSMS, embryoid bodies, mouse, myogenesis, affinity purification, nuclear extract

Rita CR Perlingeiro
contact affiliation	Department of Medicine, Cardiology division, Lillehei Heart Institute, University of Minnesota, USA
contact email	perli032@umn.edu
lab head
Alessandro Magli
contact affiliation	University of Minnesota Dept of Medicine Cardiology division Twin Cities campus Minneapolis
contact email	alemagli@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012693

PRIDE project URI

• PRIDE
  1. PXD012693
     1. Label: PRIDE project
     2. Name: Pax3 interactome in mouse ES cell-derived mesodermal precursors