PXD012684

PXD012684 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of Hordeum vulgare epidermis containing haustoria from Blumeria graminis f.sp. hordei
Description	Blumeria graminis f.sp. hordei is an obligate biotrohic fungal pathogen causing powdery mildew in barley. As for other biotrophic fungi, haustorial structures are at the centre of the biotrophic interaction and molecular exchanges, delivering fungal effectors or virulence factors, and taking nutrient from the host. Haustoria are originiated by the fungus, following successful penetration of the initial penetration peg through the plant cell call. Haustorial structures mainly of fungal origin, but they are surrounding by a plant component, the extrauhaustorial membrane and matrix (EHM and EHMx) forming the extrahuastorial complex (EHMc). The plant protein make-up of the plant extrahaustorial components remained unexplored, and this is a first study trying to describe plant proteome associated with haustoria using samples enriched for these structures. Therefore, proteomes of haustoria enriched samples from the epidermis of barley leaves infected with Blumeria graminins f.sp. hordei, the causing agent of barley powdery mildew, were compared to infected epidermis and un-infected epidermis to identify haustoria associated plant proteins. Haustoria were enriched from infected epidermis by digesting epidermal cell walls with cell wall degrading enzymes prior to enrichment for haustorial structures. Proteins identified in these samples were compared to infected and uninfected epidermis samples using a non-targeted label free semi-quantitation method.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:04.684.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Lambertucci
SpeciesList	scientific name: Hordeum vulgare subsp. vulgare; NCBI TaxID: 112509;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-13 02:28:44	ID requested
1	2019-11-01 05:07:30	announced
⏵ 2	2023-11-14 08:50:05	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: haustorium pl. haustoria, extrahaustorial complex, barley,Hordeum vulgare, Blumeria graminins f.sp. hordei, extrahaustorial membrane, epidermis, extrahaustorial matrix, cereals, powdery mildew, biotrophic plant pathogen, plant disease, fungus, biotic stress

Laurence Bindschedler
contact affiliation	Royal Holloway, University of London
contact email	Laurence.Bindschedler@rhul.ac.uk
lab head
Sebastien Lambertucci
contact affiliation	RHUL
contact email	Zyva096@live.rhul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012684

PRIDE project URI

• PRIDE
  1. PXD012684
     1. Label: PRIDE project
     2. Name: LC-MSMS of Hordeum vulgare epidermis containing haustoria from Blumeria graminis f.sp. hordei