PXD012682

PXD012682 is an original dataset announced via ProteomeXchange.

Title	Cleavable Hydrophobic Derivatization Strategy for Enrichment and Identification of Phosphorylated Lysine Peptides
Description	Herein, a cleavable hydrophobic derivatization (CHD) strategy was established for the enrichment and identification of pLys peptides. By CHD, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate (Dec-disulf-NHS) was synthesized to react with lysine dephosphorylated peptides, and then the derived peptides were captured by a C18 column, followed by the cleavage of hydrophobic chain to leave the specific label on the target peptides.
HostingRepository	iProX
AnnounceDate	2019-02-12
AnnouncementXML	Submission_2019-12-10_22:08:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yechen Hu
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-12 18:58:59	ID requested
1	2019-02-12 19:03:19	announced
⏵ 2	2019-12-10 22:08:43	announced	2019-12-11: Update publication information.

Hu Y, Li Y, Gao H, Jiang B, Zhang X, Li X, Wu Q, Liang Z, Zhang L, Zhang Y, Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides. Anal Bioanal Chem, 411(18):4159-4166(2019) [pubmed]

submitter keyword: Phosphorylated Lysine peptides, Hydrophobic Derivatization, N-phosphorylation, Proteomics, LC-MS/MS

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
Yechen Hu
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	yechenhu@dicp.ac.cn
dataset submitter

iProX dataset URI