PXD012680

PXD012680 is an original dataset announced via ProteomeXchange.

Title	Identification of FET-oncoprotein-interacting proteins by immunoprecipitation and mass spectrometry analysis in sarcoma cell lines
Description	The FET family of fusion oncogenes carry one of the three genes, FUS, EWSR1 or TAF15, as 5’‑partners juxtaposed to one of many different DNA binding transcription factor genes as 3’‑partners. FET fusion oncogenes are pathognomonic for many types of sarcoma and leukemia, such as FUS-DDIT3 in myxoid liposarcoma (MLS) and EWSR1-FLI1 in Ewing sarcoma (EWS). We used recombinant FET N-terminal domains in a pulldown screen to find interaction partners to the FET proteins and identified components of the SWI/SNF complex. The ~2 MDa SWI/SNF chromatin remodeling complex utilizes energy from ATP hydrolysis to remodel nucleosomes and expose DNA. We used immunoprecipitation followed by mass spectrometry or western blot analysis to extensively characterize the interaction between FET fusion oncoproteins and the SWI/SNF complex.
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-04-12_01:39:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sahlgrenska Academy Proteomics Core
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; LTQ FT; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-12 07:38:41	ID requested
1	2019-03-12 19:21:35	announced
⏵ 2	2019-04-12 01:39:11	announced	Updated publication reference for PubMed record(s): 30962207.

Lind, é, n M, Thomsen C, Grundevik P, Jonasson E, Andersson D, Runnberg R, Dolatabadi S, Vannas C, Luna Santamar, ί, a M, Fagman H, St, å, hlberg A, Å, man P, FET family fusion oncoproteins target the SWI/SNF chromatin remodeling complex. EMBO Rep, 20(5):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Sarcoma, immunoprecipitation, LC-MS, FT ICR, Orbitrap

Malin Linden
contact affiliation	Sahlgrenska Academy, University of Gothenburg, Sweden
contact email	malin.linden@gu.se
lab head
Sahlgrenska Academy Proteomics Core
contact affiliation	University of Gothenburg
contact email	egor.vorontsov@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012680

PRIDE project URI

• PRIDE
  1. PXD012680
     1. Label: PRIDE project
     2. Name: Identification of FET-oncoprotein-interacting proteins by immunoprecipitation and mass spectrometry analysis in sarcoma cell lines