PXD012652
PXD012652 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Anti-inflammatory Role of Curcumin in LPS Treated A549 cells at Global Proteome level and on Mycobacterial infection. |
| Description | A diet derived agent Curcumin (Diferuloylmethane), demonstrated its clinical application in inflammation, infection and cancer conditions. Nevertheless, its impact on the proteome of epithelial cells of non-small cell lung carcinoma (NSCLC) still needs further attention. We employed a stable isotope labeling method for cell culture (SILAC) based relative quantitative proteomics and informatics analysis to comprehend global proteome change in A549 cells treated to curcumin and/or Lipopolysaccharide (LPS). Pretreated A549 cells were infected with Mycobacterium tuberculosis H37Rv strain to monitor bacterial entry and load. With exposure to curcumin and LPS, out of the 1492 identified proteins, 305 and 346 proteins showed deregulation respectively. The expression of BID and AIFM1 mitochondrial proteins which play critical role in apoptotic pathway was deregulated in curcumin treated cells. A549 cells treated with curcumin showed higher active mitochondria intensity with respect to LPS treatment. Simultaneous treatment of curcumin and LPS neutralized the effect of LPS. Infection of pretreated A549 cells with Mycobacterial H37Rv strain, showed successful internalization with varied bacterial load. Treatment with both curcumin and LPS had similar bacterial load as control cells. This study generated evidence to deepen our understanding on anti-inflammatory role of curcumin and identified targets might be useful as drug targets. |
| HostingRepository | MassIVE |
| AnnounceDate | 2019-02-21 |
| AnnouncementXML | Submission_2019-02-21_23:57:03.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rakesh Arya |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | pyrophospho; Carbamidomethyl |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-02-10 05:00:34 | ID requested | |
| ⏵ 1 | 2019-02-21 23:57:04 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: A549, Curcumin, LPS, SILAC, LTQ-Orbitrap, LC-MS/MS, MaxQuant |
Contact List
| Dr. Ranjan Kumar Nanda | |
|---|---|
| contact affiliation | International Center for Genetic Engineering and Biotechnology, New Delhi, INDIA |
| contact email | ranjan@icgeb.res.in |
| lab head | |
| Rakesh Arya | |
| contact affiliation | ICGEB |
| contact email | rakesharya@icgeb.res.in |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083428 |
to receive all new ProteomeXchange dataset release announcements!
