PXD012645

PXD012645 is an original dataset announced via ProteomeXchange.

Title	BALF proteomics
Description	Lung cancer is one of the most common cancers and the leading cause of cancer-related mortality. Because the early diagnosis of the cancer is one of the major goals in lung cancer research, the molecule-based sensitive detection of biomarkers from bronchoalveolar lavage fluid (BALF) to diagnose the lung cancer has been suggested as a promising method. BALF is a fluid that can be easily obtained from patients with lung diseases and the process of collecting the fluid is relatively cheap and non-invasive. Here, we developed a novel method for in-depth single proteomic analysis of BALF by using antibody-based depletion of high abundant proteins from BALF. We identified, in total, 4,615 protein groups mapped to 4,535 gene names using LC-MS/MS. We found our method outperformed conventional methods. With the comprehensive result, we believe that this method would facilitate lung cancer biomarker discovery.
HostingRepository	PRIDE
AnnounceDate	2019-05-30
AnnouncementXML	Submission_2019-05-30_01:16:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seo-Young Sim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 06:53:41	ID requested
⏵ 1	2019-05-30 01:16:12	announced

Sim SY, Choi YR, Lee JH, Lim JM, Lee SE, Kim KP, Kim JY, Lee SH, Kim MS, In-Depth Proteomic Analysis of Human Bronchoalveolar Lavage Fluid toward the Biomarker Discovery for Lung Cancers. Proteomics Clin Appl, 13(5):e1900028(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: BALF, lung cancer, depletion

Min-Sik Kim
contact affiliation	Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Republic of Korea
contact email	mkim@dgist.ac.kr
lab head
Seo-Young Sim
contact affiliation	Kyung-Hee university
contact email	aliceblood@naver.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012645

PRIDE project URI

• PRIDE
  1. PXD012645
     1. Label: PRIDE project
     2. Name: BALF proteomics